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Anti-KLF4 Antibody (aa2-470) LS-C149986

Ordering

Wt. Vol. Conc. Price
100 µg - 0.2 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular KLF4 Antibodies

Anti-KLF4 Antibody (aa1-170, clone AT4E6) IHC-plus™ LS-B2966
Mouse Monoclonal [clone AT4E6] (IgG1,k) to Human KLF4
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-KLF4 Antibody (aa401-450) IHC-plus™ LS-B5641
Rabbit Polyclonal (IgG) to Human KLF4
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Zebrafish, Drosophila
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-KLF4 Antibody (aa2-470) LS-C149986
Goat Polyclonal (IgG) to Human KLF4
Human
IHC - Paraffin, ICC, Western blot, Chromatin Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human KLF4
Human
IHC - Paraffin, ICC, Western blot, Chromatin Immunoprecipitation
Unconjugated

Details

Human KLF4
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (15 µg/ml)
  • ICC (5 - 15 µg/ml)
  • Western blot (0.5 µg/ml)
  • Chromatin Immunoprecipitation

Specificity and Use

KLF4 antibody was raised against e. coli-derived recombinant human KLF4 Ala2-Phe470 Accession # AAH29923.
aa2-470
Detects human KLF4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately.
Western Blot, 0.5 µg/ml, Chromatin Immunoprecipitation (ChIP), 5 ug/5 x 106 cells. Immunocytochemistry, 5-15 µg/ml. Immunohistochemistry, 15 µg/ml.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Reconstitute with 500ul sterile PBS
Store at -20 to -70 C. Stable for 12 months from date of receipt.
For research use only.

About KLF4

O43474 NM_004235 NP_004226.3

KLF4 Antibody, EZF Antibody, GKLF Antibody, Kruppel-like factor 4 (gut) Antibody, Hezf Antibody, Krueppel-like factor 4 Antibody

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface.

Immunohistochemistry

Immunohistochemistry
KLF4 in Human Colon. KLF4 was detected in immersion fixed paraffin-embedded sections of human colon using 15 ug/ml Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Tissue was stained with Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to the nuclei of epithelial cells.

Immunocytochemistry

Immunocytochemistry
KLF4 in BG01 V Human Stem Cells. KLF4 was detected in immersion fixed BG01 V human embryonic stem cells using 10 ug/ml Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody for 3 hours at room temperature. Cells were stained with the NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).

Western blot

Western blot
Detection of Human KLF4 by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, SW480 human bladder cancer cell line, and HCT-116 human colorectal carcinoma cell line. PVDF membrane was probed with 0.5 ug/ml of Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for KLF4 at approximately 60 kD (as indicated). This experiment was conducted under reducing conditions.

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation
Detection of KLF4-regulated Genes by Chromatin Immunoprecipitation. BG01 V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. KLF4/DNA complexes were immunoprecipitated using 5 ug Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody or control antibody for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody. immune complexes were captured using 50 ul of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The B2R promoter was detected by standard PCR.

Requested From: United States
Date Requested: 12/11/2016

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