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Anti-JAG1 / Jagged 1 Antibody (aa32-296) LS-C149984

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular JAG1 / Jagged 1 Antibodies

Anti-JAG1 / Jagged 1 Antibody (aa110-125) LS-C71129
Rabbit Polyclonal (IgG) to Human JAG1 / Jagged 1
Human, Mouse, Rat
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-JAG1 / Jagged 1 Antibody (aa110-125) IHC-plus™ LS-B2442
Rabbit Polyclonal (IgG) to Human JAG1 / Jagged 1
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human JAG1 / Jagged 1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human JAG1 / Jagged 1
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (1 µg/ml)
  • ELISA (15 µg/ml)

Specificity and Use

JAG1 / Jagged 1 antibody was raised against mouse myeloma cell line NS0-derived recombinant human Jagged 1 Ser32-Asp296 Accession # P78504.
aa32-296
Detects human Jagged 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat.
Western Blot, 1 µg/ml, Huh-7 human hepatoma cell line and HepG2 human hepatocellular carcinoma cell line. Immunohistochemistry, 5-15 µg/ml, Blockade of Receptor-ligand Interaction, In a functional ELISA, 3-15 µg/ml of this antibody will block 50% of the binding of 200 ng/ml of Recombinant Rat. Jagged 1 Fc Chimera to immobilized Recombinant Rat Notch-1 coated at 5 µg/ml (100 ul/well). At 100 µg/ml, this antibody will block >90% of the binding.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About JAG1 / Jagged 1

P78504 NM_000214 NP_000205.1

JAG1 Antibody, AGS Antibody, AHD Antibody, Alagille syndrome Antibody, CD339 antigen Antibody, HJ1 Antibody, Jagged1 Antibody, JAGL1 Antibody, Jagged Antibody, Jagged 1 Antibody, Protein jagged-1 Antibody, AWS Antibody, CD339 Antibody, Soluble protein jagged Antibody

Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).

Immunohistochemistry

Immunohistochemistry
Jagged 1 in Human Kidney Cancer Tissue. Jagged 1 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4°C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).

Western blot

Western blot
Detection of Human Jagged 1 by Western Blot. Western blot shows lysates of Huh7 human hepatoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 ug/ml of Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for Jagged 1 at approximately 180 kD (as indicated). This experiment was conducted under reducing conditions.

Requested From: United States
Date Requested: 12/10/2016

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