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Catalog Number Size Price
LS-C18929-500 500 µg (1 mg/ml) $541 
JAG1 / Jagged 1 Antibody - Immunohistochemistry - Jagged-1 Antibody.
JAG1 / Jagged 1 Antibody - Immunofluorescence - Jagged-1 Antibody. Immunofluorescence microscopy using Protein A purified anti-Jagged-1 antibody of human corneal epithelial cells. Primary antibody was used at a 1:500 dilution. The Jagged1 (green staining) is localized to the cytoplasm and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (blue). Personal Communication. Aihua Ma, Univdersity of Cardiff.
JAG1 / Jagged 1 Antibody - Anti-Jagged-1 Antibody - Western Blot. Western blot of Protein A purified anti-Jagged-1 antibody shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at ~134 kD in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG. Exposure time was 1 min. Predicted molecular weight is 134 kD.
JAG1 / Jagged 1 Antibody - Immunohistochemistry - Jagged-1 Antibody.
JAG1 / Jagged 1 Antibody - Immunofluorescence - Jagged-1 Antibody. Immunofluorescence microscopy using Protein A purified anti-Jagged-1 antibody of human corneal epithelial cells. Primary antibody was used at a 1:500 dilution. The Jagged1 (green staining) is localized to the cytoplasm and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (blue). Personal Communication. Aihua Ma, Univdersity of Cardiff.
JAG1 / Jagged 1 Antibody - Anti-Jagged-1 Antibody - Western Blot. Western blot of Protein A purified anti-Jagged-1 antibody shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at ~134 kD in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG. Exposure time was 1 min. Predicted molecular weight is 134 kD.
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Polyclonal Rabbit anti‑Human JAG1 / Jagged 1 Antibody (N‑Terminus, IHC, IF, WB) LS‑C18929

Polyclonal Rabbit anti‑Human JAG1 / Jagged 1 Antibody (N‑Terminus, IHC, IF, WB) LS‑C18929

Antibody:
JAG1 / Jagged 1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$541
LS-C18929-500
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
JAG1 / Jagged 1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Jagged 1 antibody LS-C18929 is an unconjugated rabbit polyclonal antibody to Jagged 1 (JAG1) (N-Terminus) from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB.
Target
Human JAG1 / Jagged 1
Synonyms
JAG1 | AGS | AHD | Alagille syndrome | CD339 antigen | HJ1 | Jagged1 | JAGL1 | Jagged | Jagged 1 | Protein jagged-1 | AWS | CD339 | Soluble protein jagged
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
This protein A purified Jagged 1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the N-Terminus of human Jagged-1 protein.
Epitope
N-Terminus
Specificity
A BLAST analysis was used to suggest cross reactivity with Jagged-1 protein from human, chimpanzee, rat and mouse based on 100% homology with the immunizing sequence. Partial reactivity is expected against dog (81%) and Xenopus laevis (85%) based on partial sequence homologies as indicated. Reactivity against homologues from other sources is not known.
Applications
  • IHC (1:100 - 1:500)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:20000 - 1:100000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This protein A purified antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 150 kD in size corresponding to Jagged-1 in mouse liver whole cell lysates, and a 75 kD band in human brain and kidney lysates.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About JAG1 / Jagged 1
P78504 NM_000214 NP_000205.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022