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Proteins > JAG1 / Jagged 1 > LS-G3788
 
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Catalog Number Size Price
LS-G3788-10 10 µg $347 
LS-G3788-50 50 µg (0.5 mg/ml) $470 
JAG1 / Jagged 1 Protein - Adipogenesis inhibition of 3T3L-1 cells. 3T3L1 cells were maintained in DMEM supplemented with 10% FBS and penicillin-streptomycin. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1 uM, and 10 ug/ml, respectively and continued for 2 days (day 0). The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml of human Jagged1-Fc and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.
JAG1 / Jagged 1 Protein - Adipogenesis inhibition. Macroscopic pictures.
JAG1 / Jagged 1 Protein - Induction of Hes-1 with the treatment of recombinant human Jagged1-Fc (Prod. No. AG-40A-0081). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human Jagged1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-human Hes1 or GAPDH. Apparently, for Notch ligands or receptors it is normally coated. Otherwise the proteins would need to be artificially multimerized. Since human and mouse Jagged-1 are almost identical (>96%) human works on mouse cells and vice versa.
JAG1 / Jagged 1 Protein - Adipogenesis inhibition of 3T3L-1 cells. 3T3L1 cells were maintained in DMEM supplemented with 10% FBS and penicillin-streptomycin. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1 uM, and 10 ug/ml, respectively and continued for 2 days (day 0). The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml of human Jagged1-Fc and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.
JAG1 / Jagged 1 Protein - Adipogenesis inhibition. Macroscopic pictures.
JAG1 / Jagged 1 Protein - Induction of Hes-1 with the treatment of recombinant human Jagged1-Fc (Prod. No. AG-40A-0081). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human Jagged1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-human Hes1 or GAPDH. Apparently, for Notch ligands or receptors it is normally coated. Otherwise the proteins would need to be artificially multimerized. Since human and mouse Jagged-1 are almost identical (>96%) human works on mouse cells and vice versa.
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Human JAG1 / Jagged 1 Protein (Recombinant Human IgG1 Fc) - LS-G3788

Human JAG1 / Jagged 1 Protein (Recombinant Human IgG1 Fc) - LS-G3788

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Description:
JAG1 / Jagged 1 Protein LS-G3788 is a Recombinant Human JAG1 / Jagged 1 produced in HEK 293 Cells aa1-1067 with Human IgG1 Fc tag(s). It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method). For Research Use Only
Price
Catalog Number
$347
LS-G3788-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Publications Reviews Images Popular JAG1 / Jagged 1 Proteins Request SDS/MSDS

Overview

Description:
JAG1 / Jagged 1 Protein LS-G3788 is a Recombinant Human JAG1 / Jagged 1 produced in HEK 293 Cells aa1-1067 with Human IgG1 Fc tag(s). It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method). For Research Use Only

Specifications

Type
Recombinant Protein
Target
JAG1 / Jagged 1
Synonyms
JAG1 | AGS | AHD | Alagille syndrome | CD339 antigen | HJ1 | Jagged1 | JAGL1 | Jagged | Jagged 1 | Protein jagged-1 | AWS | CD339 | Soluble protein jagged
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Tag
Human IgG1 Fc
Region
aa1-1067
Predicted Molecular Weight
~150kDa (SDS-PAGE)
Expression System
HEK 293 Cells
Source Species
Human
Purification
Greater than 90% by SDS-PAGE
Bio-Activity
Inhibits adipogenesis of mesenchymal stem cells (MSCs). Induces Hes-1 in 3T3L-1 cells.
Endotoxin
Less than 0.1 EU/µg protein (determined by LAL method).
Presentation
PBS
Storage
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About JAG1 / Jagged 1
P78504 NM_000214 NP_000205.1

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Images

Functional Assay

JAG1 / Jagged 1 Protein - Adipogenesis inhibition of 3T3L-1 cells. 3T3L1 cells were maintained in DMEM supplemented with 10% FBS and penicillin-streptomycin. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1 uM, and 10 ug/ml, respectively and continued for 2 days (day 0). The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml of human Jagged1-Fc and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.
Adipogenesis inhibition of 3T3L-1 cells. 3T3L1 cells were maintained in DMEM supplemented with 10% FBS and penicillin-streptomycin. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1 uM, and 10 ug/ml, respectively and continued for 2 days (day 0). The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml of human Jagged1-Fc and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.

Functional Assay

JAG1 / Jagged 1 Protein - Adipogenesis inhibition. Macroscopic pictures.
Adipogenesis inhibition. Macroscopic pictures.

Functional Assay

JAG1 / Jagged 1 Protein - Induction of Hes-1 with the treatment of recombinant human Jagged1-Fc (Prod. No. AG-40A-0081). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human Jagged1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-human Hes1 or GAPDH. Apparently, for Notch ligands or receptors it is normally coated. Otherwise the proteins would need to be artificially multimerized. Since human and mouse Jagged-1 are almost identical (>96%) human works on mouse cells and vice versa.
Induction of Hes-1 with the treatment of recombinant human Jagged1-Fc (Prod. No. AG-40A-0081). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human Jagged1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-human Hes1 or GAPDH. Apparently, for Notch ligands or receptors it is normally coated. Otherwise the proteins would need to be artificially multimerized. Since human and mouse Jagged-1 are almost identical (>96%) human works on mouse cells and vice versa.

Functional Assay

JAG1 / Jagged 1 Protein - Adipogenesis inhibition of 3T3L-1 cells. 3T3L1 cells were maintained in DMEM supplemented with 10% FBS and penicillin-streptomycin. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1 uM, and 10 ug/ml, respectively and continued for 2 days (day 0). The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml of human Jagged1-Fc and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.
Adipogenesis inhibition of 3T3L-1 cells. 3T3L1 cells were maintained in DMEM supplemented with 10% FBS and penicillin-streptomycin. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1 uM, and 10 ug/ml, respectively and continued for 2 days (day 0). The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml of human Jagged1-Fc and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.

Functional Assay

JAG1 / Jagged 1 Protein - Adipogenesis inhibition. Macroscopic pictures.
Adipogenesis inhibition. Macroscopic pictures.

Functional Assay

JAG1 / Jagged 1 Protein - Induction of Hes-1 with the treatment of recombinant human Jagged1-Fc (Prod. No. AG-40A-0081). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human Jagged1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-human Hes1 or GAPDH. Apparently, for Notch ligands or receptors it is normally coated. Otherwise the proteins would need to be artificially multimerized. Since human and mouse Jagged-1 are almost identical (>96%) human works on mouse cells and vice versa.
Induction of Hes-1 with the treatment of recombinant human Jagged1-Fc (Prod. No. AG-40A-0081). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human Jagged1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-human Hes1 or GAPDH. Apparently, for Notch ligands or receptors it is normally coated. Otherwise the proteins would need to be artificially multimerized. Since human and mouse Jagged-1 are almost identical (>96%) human works on mouse cells and vice versa.

Functional Assay

JAG1 / Jagged 1 Protein - Adipogenesis inhibition of 3T3L-1 cells. 3T3L1 cells were maintained in DMEM supplemented with 10% FBS and penicillin-streptomycin. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1 uM, and 10 ug/ml, respectively and continued for 2 days (day 0). The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml of human Jagged1-Fc and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.
Adipogenesis inhibition of 3T3L-1 cells. 3T3L1 cells were maintained in DMEM supplemented with 10% FBS and penicillin-streptomycin. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1 uM, and 10 ug/ml, respectively and continued for 2 days (day 0). The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml of human Jagged1-Fc and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.

Functional Assay

JAG1 / Jagged 1 Protein - Adipogenesis inhibition. Macroscopic pictures.
Adipogenesis inhibition. Macroscopic pictures.

Functional Assay

JAG1 / Jagged 1 Protein - Induction of Hes-1 with the treatment of recombinant human Jagged1-Fc (Prod. No. AG-40A-0081). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human Jagged1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-human Hes1 or GAPDH. Apparently, for Notch ligands or receptors it is normally coated. Otherwise the proteins would need to be artificially multimerized. Since human and mouse Jagged-1 are almost identical (>96%) human works on mouse cells and vice versa.
Induction of Hes-1 with the treatment of recombinant human Jagged1-Fc (Prod. No. AG-40A-0081). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human Jagged1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-human Hes1 or GAPDH. Apparently, for Notch ligands or receptors it is normally coated. Otherwise the proteins would need to be artificially multimerized. Since human and mouse Jagged-1 are almost identical (>96%) human works on mouse cells and vice versa.

Functional Assay

JAG1 / Jagged 1 Protein - Adipogenesis inhibition of 3T3L-1 cells. 3T3L1 cells were maintained in DMEM supplemented with 10% FBS and penicillin-streptomycin. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1 uM, and 10 ug/ml, respectively and continued for 2 days (day 0). The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml of human Jagged1-Fc and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.
Adipogenesis inhibition of 3T3L-1 cells. 3T3L1 cells were maintained in DMEM supplemented with 10% FBS and penicillin-streptomycin. When the cells were confluently grown adipogenesis was initiated by adding IBMX, Dexamethasone, and insulin to 0.5mM, 1 uM, and 10 ug/ml, respectively and continued for 2 days (day 0). The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml of human Jagged1-Fc and human CD137-Fc as a control-Fc. Staining with Oil Red O was typically performed on day 7.

Functional Assay

JAG1 / Jagged 1 Protein - Adipogenesis inhibition. Macroscopic pictures.
Adipogenesis inhibition. Macroscopic pictures.

Functional Assay

JAG1 / Jagged 1 Protein - Induction of Hes-1 with the treatment of recombinant human Jagged1-Fc (Prod. No. AG-40A-0081). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human Jagged1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-human Hes1 or GAPDH. Apparently, for Notch ligands or receptors it is normally coated. Otherwise the proteins would need to be artificially multimerized. Since human and mouse Jagged-1 are almost identical (>96%) human works on mouse cells and vice versa.
Induction of Hes-1 with the treatment of recombinant human Jagged1-Fc (Prod. No. AG-40A-0081). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human Jagged1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-human Hes1 or GAPDH. Apparently, for Notch ligands or receptors it is normally coated. Otherwise the proteins would need to be artificially multimerized. Since human and mouse Jagged-1 are almost identical (>96%) human works on mouse cells and vice versa.

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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