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Anti-IL-10 Antibody (clone 15B9-1-2) LS-C104515


Wt. Vol. Conc. Price
500 µg 500 µl - $255
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular IL-10 Antibodies

Anti-IL-10 Antibody (clone JES5-2A5) IHC-plus™ LS-B4913
Rat Monoclonal [clone JES5-2A5] (IgG1) to Mouse IL-10
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal [clone 15B9-1-2] (IgG2a) to Mouse IL-10
Western blot, ELISA, Neutralization


Mouse IL-10
Mouse (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [15B9-1-2]
Protein G purified


  • Western blot (1 - 2 µg/ml)
  • ELISA (4 - 8 µg/ml)
  • Neutralization (3 - 5 µg/ml)

Specificity and Use

IL-10 antibody was raised against e.coli derived recombinant Murine IL-10.
Mouse IL-10
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Murine IL-10 (0.5 ng/ml), a concentration of 3-5 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 4-8 ug/ml of this antibody will detect at least 1600 pg/ml of recombinant murine IL-10 when used with biotinylated antigen affinity purified anti-murine IL-10 (LS-C104822) as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1-2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2-4 ng/lane, under reducing or non-reducing conditions.


Lyophilized from PBS
500 µl Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About IL-10

P22301 NM_000572 NP_000563.1

IL10 Antibody, CSIF Antibody, GVHDS Antibody, IL-10 Antibody, IL10A Antibody, Interleukin-10 Antibody, Interleukin 10 Antibody, TGIF Antibody

IL-10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway.

Western blot

Western blot
Western Blot (reducing) of IL-10 antibody LS-C104515

Western blot

Western blot
Western Blot (non-reducing) of IL-10 antibody LS-C104515

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of IL-10 antibody LS-C104515

Requested From: United States
Date Requested: 10/27/2016

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