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Anti-IL-10 Antibody (Biotin) LS-C104321

Note: This antibody replaces LS-C8092, LS-C8103, LS-C43004, LS-C54080

Ordering

Wt. Vol. Conc. Price
25 µg - - $285
50 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL-10 Antibodies

Anti-IL-10 Antibody (clone JES5-2A5) IHC-plus™ LS-B4913
Rat Monoclonal [clone JES5-2A5] (IgG1) to Mouse IL-10
Mouse
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human IL-10
Human
Western blot, ELISA
Biotin Conjugated

Details

Human IL-10
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Biotin
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (1 µg/ml)

Specificity and Use

IL-10 antibody was raised against e.coli derived recombinant Human IL-10.
Human IL-10
ELISA: To detect hIL-10 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-10. To detect hIL-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-10 (LS-B7432) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-10. Western Blot: To detect hIL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-10 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

Lyophilized from PBS, pH 7.2
Sterile PBS containing 0.1% BSA
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About IL-10

P22301 NM_000572 NP_000563.1

IL10 Antibody, CSIF Antibody, GVHDS Antibody, IL-10 Antibody, IL10A Antibody, Interleukin-10 Antibody, Interleukin 10 Antibody, TGIF Antibody

IL-10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of IL-10 antibody LS-C104321 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Direct ELISA of IL-10 antibody LS-C104321 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/10/2016

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