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Anti-HSPG2 / Perlecan Antibody (clone A7L6) LS-C15760

Ordering

Wt. Vol. Conc. Price
100 µg - - $530
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular HSPG2 / Perlecan Antibodies

Anti-HSPG2 / Perlecan Antibody (clone A7L6) LS-C15761
Rat Monoclonal [clone A7L6] (IgG2a,k) to Mouse HSPG2 / Perlecan
Mouse, Human, Bovine, Pig
IHC - Frozen, Immunofluorescence
Unconjugated
Anti-HSPG2 / Perlecan Antibody (clone A71) IHC-plus™ LS-B2468
Mouse Monoclonal [clone A71] (IgG1,k) to Bovine HSPG2 / Perlecan
Bovine, Human
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal [clone A7L6] (IgG2a,k) to Mouse HSPG2 / Perlecan
Mouse, Human, Bovine
ICC
Unconjugated

Details

Mouse HSPG2 / Perlecan
Rat
Mouse, Human, Bovine (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [A7L6]
Unconjugated
Protein G purified
Unmodified

Applications

ICC (10 µg/ml)

Specificity and Use

HSPG2 / Perlecan antibody was raised against murine EHS laminin preparation containing entactin and heparan sulfate proteoglycan.
Recognizes mouse low density basement membrane heparan sulfate proteoglycan (perlecan) core protein. Does not cross-react with other basement membrane proteins or fibronectin. Species cross-reactivity: Human and bovine.
Suitable for use in Immunocytochemistry. Immunocytochemistry: 10 ug/ml on primary cultures of hippocampal neurons, fixed with 4% paraformaldehyde in phosphate buffer containing 4% sucrose. The neurons were subsequently permeabilized with 0.25% Triton X-100 for 5 minutes at RT. The fixed and permeabilized cells were blocked with normal donkey serum in Tris buffered saline (TBS) containing 10% Tween. The primary antibody was detected using a FITC-conjugated goat anti-rat secondary antibody.

Packaging

0.1 M Tris-glycine, pH 7.4, 0.15 sodium chloride, 0.05% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About HSPG2 / Perlecan

P98160 NM_005529 NP_005520.4

HSPG2 Antibody, Endorepellin (domain V region) Antibody, HSPG Antibody, Heparan sulfate proteoglycan 2 Antibody, Perlecan Antibody, Perlecan proteoglycan Antibody, SJA Antibody, SJS1 Antibody, PRCAN Antibody, SJS Antibody

Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury.

Requested From: United States
Date Requested: 12/4/2016

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