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Anti-HMG1 / HMGB1 Antibody (clone HMG1-5H6) LS-C188707


Wt. Vol. Conc. Price
100 µg - 1 mg/ml Unavailable

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100% Guaranteed 100% Guaranteed
Hamster Monoclonal [clone HMG1-5H6] (IgG) to Mouse HMG1 / HMGB1
Mouse, Human, Rat, Hamster
Immunofluorescence, Western blot, Flow Cytometry


Mouse HMG1 / HMGB1
Mouse, Human, Rat, Hamster (tested or 100% immunogen sequence identity)
IgG Monoclonal [HMG1-5H6]
Protein G purified


  • Immunofluorescence
  • Western blot
  • Flow Cytometry

Specificity and Use

HMG1 / HMGB1 antibody was raised against recombinant mouse HMGB1.
Recognizes High mobility group protein B1 (HMGB1), a ubiquitously expressed nuclear DNA binding protein, and one of a newly emerging group of alarmins, which acts as a stabilizer of nucleosome formation, and facilitates transcription factor binding, by bending DNA. HMGB1 is one of the signature danger signals of endogenous cellular injury, and is released outside the cell by necrotic and inflammatory cells to act as a chemoattractant for immature dendritic cells (DCs), promoting their maturation. DCs can also secrete HMGB1, promoting proliferation and Th1 polarization of interacting T cells. Cellular injury resulting in necrosis, leads to passive HMGB1 release, and microbes or pro-inflammatory cytokines may later stimulate active release from APCs. HMGB1 is emerging as a prime specific marker and regulator of necrotic cell death, possibly through the PI3KC3-MEK-ERK pathway. It interacts directly with the autophagy protein Beclin-1, and binds to receptors, such as RAGE on endothelial cells, and Toll-like receptors on macrophages. Studies identifying microtubule-associated protein 1 light chain 3 (LC3) lipidation and redistribution, coupled with the accumulation of autophagosomes and autolysosomes, have shown an important role for HMGB1 release in sustaining autophagy. Studies have also shown that HMGB1 released after chemotherapy treatment is a critical regulator of autophagy, and a potential drug target for therapeutic interventions in leukemia. HMGB1 is increasingly recognized as an important protein in medical research. It is angiogenic and promotes cardiac stem cell growth and differentiation, it may act as an adjuvant or assist in tissue repair, and is also a prototypical damage-associated molecular pattern molecule (DAMP) which co-precipitates with CD24 and is associated with the hallmarks of cancer. Accelerated levels of HMGB1 are seen in several cancers and correlates with metastatic activity. Studies have shown that clone HMG1-5H6 cross-reacts with human, rat and hamster HMGB1.


PBS, 0.09% sodium azide
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About HMG1 / HMGB1

P09429 NM_002128 NP_002119.1

HMGB1 Antibody, Amphoterin Antibody, High mobility group protein B1 Antibody, High mobility group box 1 Antibody, High mobility group protein 1 Antibody, HMG1 Antibody, HMG3 Antibody, SBP-1 Antibody, High-mobility group box 1 Antibody, HMG-1 Antibody

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Requested From: 
Date Requested: 4/22/2017

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