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Anti-HMG1 / HMGB1 Antibody (clone 2F6) IHC-plus™ LS-B4344

Note: This antibody replaces LS-C104968

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HMG1 / HMGB1 Antibodies

Anti-HMG1 / HMGB1 Antibody IHC-plus™ LS-B2841
Rabbit Polyclonal (IgG) to Pig HMG1 / HMGB1
Pig, Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HMG1 / HMGB1 Antibody (clone 1D5) IHC-plus™ LS-B4341
Mouse Monoclonal [clone 1D5] (IgG2a,k) to Human HMG1 / HMGB1
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-HMG1 / HMGB1 Antibody (aa25-75) LS-C148759
Rabbit Polyclonal to Human HMG1 / HMGB1
Human, Monkey, Mouse, Rat, Chicken
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 2F6] (IgG2a,k) to Human HMG1 / HMGB1
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human HMG1 / HMGB1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [2F6]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

HMG1 / HMGB1 antibody was raised against hMGB1 (NP_002119, 1 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Cell lysate) - positive control Hela NE. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells.

Packaging

PBS, pH 7.2
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About HMG1 / HMGB1

P09429 NM_002128 NP_002119.1

HMGB1 Antibody, Amphoterin Antibody, High mobility group protein B1 Antibody, High mobility group box 1 Antibody, High mobility group protein 1 Antibody, HMG1 Antibody, HMG3 Antibody, SBP-1 Antibody, High-mobility group box 1 Antibody, HMG-1 Antibody

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Immunohistochemistry

Immunohistochemistry
Anti-HMGB1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4344 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-HMGB1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4344 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-HMGB1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4344 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to HMGB1 on HeLa cell (antibody concentration 10 ug/ml).

Western blot

Western blot
Western blot of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody, clone 2F6.

Western blot

Western blot
HMGB1 monoclonal antibody, clone 2F6 Western blot of HMGB1 expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged HMGB1 is approximately 0.3 ng/ml as a capture antibody.
 

Requested From: United States
Date Requested: 12/7/2016

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