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Anti-HMG1 / HMGB1 Antibody (aa1-215, clone J2E1) LS-C93743

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $315
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HMG1 / HMGB1 Antibodies

Anti-HMG1 / HMGB1 Antibody IHC-plus™ LS-B2841
Rabbit Polyclonal (IgG) to Pig HMG1 / HMGB1
Pig, Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HMG1 / HMGB1 Antibody (clone 1D5) IHC-plus™ LS-B4341
Mouse Monoclonal [clone 1D5] (IgG2a,k) to Human HMG1 / HMGB1
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-HMG1 / HMGB1 Antibody (aa25-75) LS-C148759
Rabbit Polyclonal to Human HMG1 / HMGB1
Human, Monkey, Mouse, Rat, Chicken
Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone J2E1] (IgG2b) to Human HMG1 / HMGB1
Human
IHC, Western blot, ELISA
Unconjugated

Details

Human HMG1 / HMGB1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [J2E1]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC
  • Western blot (1:500 - 1:1000)
  • ELISA

  • IHC - Paraffin

Specificity and Use

HMG1 / HMGB1 antibody was raised against recombinant human HMGB1 (1-215aa) purified from High Five (Trichoplusia ni) insect cells. NCBI Accession No.: NP_002119.
aa1-215
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500-1:1000.

Packaging

PBS, pH 7.4, 0.1% sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About HMG1 / HMGB1

P09429 NM_002128 NP_002119.1

HMGB1 Antibody, Amphoterin Antibody, High mobility group protein B1 Antibody, High mobility group box 1 Antibody, High mobility group protein 1 Antibody, HMG1 Antibody, HMG3 Antibody, SBP-1 Antibody, High-mobility group box 1 Antibody, HMG-1 Antibody

DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

Western blot

Western blot
Cell lysates of Jurkat and HeLa (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human HMGB1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
 

Requested From: United States
Date Requested: 12/7/2016

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