Target
Human HLA-B
Synonyms
HLA-B | HLAB | MHC Class I HLA heavy chain | Leukocyte antigen class I-B | SPDA1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Purification
Ion exchange chromatography
Immunogen
Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.
Specificity
Affinity studies by Scatchard analysis showed that HLA-B27 antibody has a higher affinity for HLA-B27 (9.7 x 10^8M-1) than for HLA B7 (9.5 x 10^7 M-1). Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative.
Usage
Suitable for use in Flow Cytometry. Flow Cytometry: Neat; Use 10 ul of suggested working dilution to label 1E6 cells or 100 ul of whole blood. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide, 0.2% BSA
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee