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Anti-HLA-B Antibody (aa62-90) LS-C164912

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HLA-B Antibodies

Anti-HLA-B Antibody (FITC) LS-C24493
Mouse Monoclonal (IgG2a) to Human HLA-B
Human
Flow Cytometry
FITC Conjugated
Anti-HLA-B Antibody (FITC) LS-C24497
Mouse Monoclonal (IgG3) to Human HLA-B
Human
Flow Cytometry
FITC Conjugated
Anti-HLA-B Antibody (clone JOAN-1) LS-C123775
Mouse Monoclonal [clone JOAN-1] (IgG1) to Human HLA-B
Human
IHC - Frozen, Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human HLA-B
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human HLA-B
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

HLA-B antibody was raised against kLH-conjugated synthetic peptide between amino acids 62-90 from N-terminal region of human HLA-B.
LS-E9677 - Lyophilized - 100 µg - $145.00
aa62-90
Human HLA-B

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About HLA-B

P01889 NM_005514 NP_005505.2

HLA-B Antibody, HLAB Antibody, MHC Class I HLA heavy chain Antibody, Leukocyte antigen class I-B Antibody, SPDA1 Antibody

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HLA-B Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot
Western blot of HLA-B Antibody in A375 cell line lysates (35 ug/lane). HLA-B (arrow) was detected using the purified antibody.

Western blot

Western blot
Western blot of HLA-B (arrow) using rabbit polyclonal HLA-B Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-B gene.

Western blot

Western blot
Western blot of lysates from CEM, HL-60, Jurkat, Ramos cell line (from left to right), using HLA-B Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Western blot

Western blot
Western blot of lysates from HL-60, Jurkat, Ramos cell line (from left to right), using HLA-B Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Western blot

Western blot
Western blot of HLA-B Antibody in A2058 cell line lysates (35 ug/lane). HLA-B (arrow) was detected using the purified antibody.

Flow Cytometry

Flow Cytometry
HLA-B Antibody flow cytometry of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
 

Requested From: United States
Date Requested: 12/4/2016

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