(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot (1 µg/ml)
- Functional Assay
- (applications tested for the base form of this product only)
Histone H3 antibody was raised against a synthetic linear peptide corresponding to monomethylated Lys9 at the N-terminal region of Histone H3 (KLH).
Recognizes human monomethylated Lys9 at and around the N-terminal of Histone H3, MW ~17kD. Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) in Western Blot analysis of HeLa acid extracts and various other modified histone peptides.
Suitable for use in Western Blot and Peptide Inhibition Assay. Western Blot: 1 ug/ml detects Histone H3 in 500 ng of recombinant Histone H3 and 10 ug of HeLa acid extract.
PBS, pH 7.2, 0.05% sodium azide
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
For research use only.
This antibody carries the LSBio 100% Guarantee.