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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Histone H3 Antibody (Mono‑Methyl‑Lys9) LS‑C129801
Antibody
Histone H3 antibody LS-C129801 is an unconjugated mouse monoclonal antibody to human Histone H3. Validated for Func and WB.
Catalog
Size
Price
LS-C129801-100
100 µg
Unavailable

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Product Description

Histone H3 antibody LS-C129801 is an unconjugated mouse monoclonal antibody to human Histone H3. Validated for Func and WB.
About Histone H3
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones.

Specifications

Target
Human Histone H3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • Functional Assay
  • (applications tested for the base form of this product only)
Immunogen
Histone H3 antibody was raised against a synthetic linear peptide corresponding to monomethylated Lys9 at the N-terminal region of Histone H3 (KLH).
Epitope
Mono-Methyl-Lys9
Specificity
Recognizes human monomethylated Lys9 at and around the N-terminal of Histone H3, MW ~17kD. Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) in Western Blot analysis of HeLa acid extracts and various other modified histone peptides.
Usage
Suitable for use in Western Blot and Peptide Inhibition Assay. Western Blot: 1 ug/ml detects Histone H3 in 500 ng of recombinant Histone H3 and 10 ug of HeLa acid extract.
Presentation
PBS, pH 7.2, 0.05% sodium azide
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/20/2018

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