(tested or 100% immunogen sequence identity)
- Western blot
Histone H2B antibody was raised against ovalbumin-conjugated, synthetic peptide APAEAcKKPAAK-C corresponding to aa12-21 of yeast histone H2B with a C-terminal cysteine added for conjugation purposes.
Recognizes yeast acetyl-Histone H2B (Lys16), Mr ~15kD. Does not react with human.
Suitable for use in Western Blot and Chromatin IP. Western Blot Analysis: 1:1000 dilution of this lot preferentially detects acetyl-histone H2B in wild-type yeast whole cell lysates as compared to lysates with a substitution mutant of K16R in histone H2B. Chromatin IP: An independent laboratory has shown that this antibody preferentially immunoprecipitates chromatin from wild type yeast, compared to yeast containing a histone H2B K16R substitution. This antibody was tested using S. cerevisiae, RPD3D strain. It has not been tested for cross-reactivity with S. pombe.
30% glycerol, 0.01% Thimerosal.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.