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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Histone H2B Antibody (Acetyl‑Lys16) LS‑C24293
Antibody
Histone H2B antibody LS-C24293 is an unconjugated rabbit polyclonal antibody to yeast Histone H2B. Validated for IP and WB.
Catalog
Size
Price
LS-C24293-100
100 µl
Unavailable

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Product Description

Histone H2B antibody LS-C24293 is an unconjugated rabbit polyclonal antibody to yeast Histone H2B. Validated for IP and WB.
About Histone H2B
Histone H2B is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the 'beads on a string' structure.

Specifications

Target
Yeast Histone H2B
Host
Rabbit
Reactivity
Yeast (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
Immunogen
Histone H2B antibody was raised against ovalbumin-conjugated, synthetic peptide APAEAcKKPAAK-C corresponding to aa12-21 of yeast histone H2B with a C-terminal cysteine added for conjugation purposes.
Epitope
Acetyl-Lys16
Specificity
Recognizes yeast acetyl-Histone H2B (Lys16), Mr ~15kD. Does not react with human.
Usage
Suitable for use in Western Blot and Chromatin IP. Western Blot Analysis: 1:1000 dilution of this lot preferentially detects acetyl-histone H2B in wild-type yeast whole cell lysates as compared to lysates with a substitution mutant of K16R in histone H2B. Chromatin IP: An independent laboratory has shown that this antibody preferentially immunoprecipitates chromatin from wild type yeast, compared to yeast containing a histone H2B K16R substitution. This antibody was tested using S. cerevisiae, RPD3D strain. It has not been tested for cross-reactivity with S. pombe.
Presentation
30% glycerol, 0.01% Thimerosal.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/18/2018

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