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Anti-HIC1 Antibody (aa396-410) LS-C186512

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $395
Inquire for larger quantities

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Most Popular HIC1 Antibodies

Anti-HIC1 Antibody (aa209-258) LS-C30180
Rabbit Polyclonal (IgG) to Human HIC1
Human, Monkey, Mouse, Rat, Guinea pig
Western blot
Unconjugated
Western blot Image
Anti-HIC1 Antibody (aa497-527, Biotin) LS-C258452
Rabbit Polyclonal (IgG) to Human HIC1
Human
Western blot, ELISA
Biotin Conjugated
Anti-HIC1 Antibody (aa497-527, PE) LS-C258455
Rabbit Polyclonal (IgG) to Human HIC1
Human
Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human HIC1
Human, Bovine, Dog, Hamster, Pig
Western blot, ELISA
Unconjugated

Details

Human HIC1
Goat
Human, Bovine, Dog, Hamster, Pig (tested or 100% immunogen sequence identity)
Guinea pig (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.5 - 1 µg/ml)
  • ELISA (1:128000)

Specificity and Use

HIC1 antibody was raised against synthetic peptide HLEGYPCPHLAYGEP from an internal region of human HIC1 (NP_006488.2; NP_001091672.1). Percent identity by BLAST analysis: Hamster, Elephant, Panda, Dog, Bovine, Pig (100%); Guinea pig (93%); Opossum (80%).
LS-E27957 - Lyophilized - 100 µg - $145.00
aa396-410
Human HIC1. This antibody is expected to recognize both reported isoforms (NP_006488.2; NP_001091672.1).
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: In transfected HEK293 transiently expressing Human HIC1 a band of approx. 100kD is observed. This band is not observed in the mock-transfected HEK293. The same band is observed endogenously in the cell lines A549 and Jurkat. The calculated molecular size is 74.5kD according to NP_006488.2. Recommended concentration, 0.5-1 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About HIC1

Q14526 NM_006497 NP_006488.2

HIC1 Antibody, Hic-1 Antibody, ZNF901 Antibody, Hypermethylated in cancer 1 Antibody, ZBTB29 Antibody

Transcriptional repressor. Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3'. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses. The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1.

Western blot

Western blot
HIC1 antibody (1 ug/ml) staining of A549 (A) and Jurkat (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Western blot
HIC1 antibody HEK293 overexpressing Human HIC1 and probed with (0.5 ug/ml) with the mock transfection in first lane. Data obtained from Dr. D. Leprince, CNRS UMR 8161, Institut de Biologie de LILLE, France

Requested From: United States
Date Requested: 12/4/2016

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