LSBio The Immunohistochemistry Antibody Company
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question?
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Contact Us

2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products. - To submit a new order. - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues. - To request technical information requests about an LSBio product or its application. - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-H2AFX / H2AX Antibody (phospho-Ser139) LS-C7424


Wt. Vol. Conc. Price
100 µg - - $455
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular H2AFX / H2AX Antibodies

Anti-H2AFX / H2AX Antibody (Ser139) IHC-plus™ LS-B1474
Rabbit Polyclonal (IgG) to Human H2AFX / H2AX
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemistry Image
Anti-H2AFX / H2AX Antibody (phospho-Ser139, clone 20E3) LS-C348689
Rabbit Monoclonal [clone 20E3] to Human H2AFX / H2AX
Human, Monkey, Mouse, Rat
IHC, Immunofluorescence, Western blot, Flow Cytometry

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1) to Human H2AFX / H2AX
Human, Monkey, Bovine, Dog, Rabbit
ICC, Immunofluorescence, Western blot, Immunoprecipitation


Human H2AFX / H2AX
Human, Monkey, Bovine, Dog, Rabbit (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Protein G purified


  • ICC (2 µg/ml)
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation

Specificity and Use

H2AFX / H2AX antibody was raised against synthetic peptide (C-KATQA[pS]QEY) corresponding to aa134-142 of human histone H2A. X. (KLH). The immunizing sequence has 8 identical amino acids in yeast and mouse.
Recognizes human Histone H2A. X phosphorylated at Ser139, Mr 15kD. A broad species cross-reactivity is expected based on conservation of sequence homology.
Suitable for use in Western Blot, Immunofluorescence, Chromatin Immunoprecipitation and Immunocytochemistry. Proteins were visualized using a IgG (HRP) GtxMo and a chemiluminescence detection system. Immunocytochemistry: 2 ug/ml detects phosphorylated histone H2A. X (Ser139) in HeLa cells treated with 0.5 uM staurosporine for 4-6 hours. Positive control: UV-treated 293 cell extracts, UV-treated HeLa cell extracts, breast cancer tissue.


PBS, pH 7.6, 0.07% sodium azide, 30% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About H2AFX / H2AX

P16104 NM_002105 NP_002096.1

H2AFX Antibody, H2AX Antibody, Histone H2A.x Antibody, H2A.X Antibody, H2A histone family, member X Antibody, H2AX histone Antibody

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Requested From: United States
Date Requested: 10/24/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number