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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GDF15 Antibody (N‑Terminus, clone 23G10.F8) LS‑C154011
GDF15 antibody LS-C154011 is an unconjugated mouse monoclonal antibody to human GDF15. Validated for ELISA and WB.
Catalog
Size
Price
LS-C154011-100
100 µg (1 mg/ml)
Unavailable

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Product Description

GDF15 antibody LS-C154011 is an unconjugated mouse monoclonal antibody to human GDF15. Validated for ELISA and WB.
About GDF15
Growth differentiation factor 15 (GDF15) is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB. GDF15 mRNA is most abundant in the liver, with lower levels seen in some other tissues. Q99988 NM_004864 NP_004855.2

GDF15 Antibody, MIC1 Antibody, MIC-1 Antibody, NAG-1 Antibody, NRG-1 Antibody, NSAID-regulated gene 1 protein Antibody, PLAB Antibody, NSAID-activated gene 1 protein Antibody, Placental TGF-beta Antibody, PTGFB Antibody, GDF-15 Antibody, PTGF-beta Antibody


Specifications

Target
Human GDF15
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [23G10.F8]
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • ELISA
Immunogen
GDF15 antibody was raised against this Protein A purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH.
Epitope
N-Terminus
Specificity
This antibody specifically reacts with the amino terminal end of human NAG-1 protein from human tissues. A BLAST analysis was used to suggest partial reactivity with NAG-1 from chimpanzee and macaque based on a 92% homology. Multimeric forms of NAG-1 may be detected. Cross-reactivity with NAG-1 from other sources has not been determined.
Usage
This Protein A purified antibody is suitable for ELISA and western blotting of human NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 C-terminal specific antibodies. This antibody is useful in dual antibody immunometric assays (EIA). Specific conditions for reactivity should be optimized by the end user. Expect bands in western blots of approximately 14 kD in size corresponding to NAG-1 monomer using the appropriate cell lysate or extract.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Anti-NAG-1 (N-terminal specific) Monoclonal Antibody - Western Blot. Western blot shows detection of recombinant NAG-1 protein (arrow) present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kD; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kD; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4°C. For detection, a 1:40000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody (LS-C60680) was used in Blocking Buffer for Fluorescent Western Blot (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc 4000MP Imaging System.
Anti-NAG-1 (N-terminal specific) Monoclonal Antibody - Western Blot. Western blot shows detection of recombinant NAG-1 protein (arrow) present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kD; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kD; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4°C. For detection, a 1:40000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody (LS-C60680) was used in Blocking Buffer for Fluorescent Western Blot (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc 4000MP Imaging System.

Western blot

Anti-NAG-1 (N-terminal specific) Monoclonal Antibody - Western Blot. Western blot shows detection of recombinant NAG-1 protein (arrow) present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kD; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kD; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4°C. For detection, a 1:40000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody (LS-C60680) was used in Blocking Buffer for Fluorescent Western Blot (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc 4000MP Imaging System.
Anti-NAG-1 (N-terminal specific) Monoclonal Antibody - Western Blot. Western blot shows detection of recombinant NAG-1 protein (arrow) present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kD; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kD; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4°C. For detection, a 1:40000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody (LS-C60680) was used in Blocking Buffer for Fluorescent Western Blot (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc 4000MP Imaging System.

Western blot

Anti-NAG-1 (N-terminal specific) Monoclonal Antibody - Western Blot. Western blot shows detection of recombinant NAG-1 protein (arrow) present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kD; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kD; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4°C. For detection, a 1:40000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody (LS-C60680) was used in Blocking Buffer for Fluorescent Western Blot (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc 4000MP Imaging System.
Anti-NAG-1 (N-terminal specific) Monoclonal Antibody - Western Blot. Western blot shows detection of recombinant NAG-1 protein (arrow) present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kD; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kD; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4°C. For detection, a 1:40000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody (LS-C60680) was used in Blocking Buffer for Fluorescent Western Blot (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc 4000MP Imaging System.

Western blot

Anti-NAG-1 (N-terminal specific) Monoclonal Antibody - Western Blot. Western blot shows detection of recombinant NAG-1 protein (arrow) present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kD; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kD; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4°C. For detection, a 1:40000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody (LS-C60680) was used in Blocking Buffer for Fluorescent Western Blot (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc 4000MP Imaging System.
Anti-NAG-1 (N-terminal specific) Monoclonal Antibody - Western Blot. Western blot shows detection of recombinant NAG-1 protein (arrow) present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kD; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kD; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. All lysates were run under reducing conditions. Primary antibody was used at a 1:1000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4°C. For detection, a 1:40000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody (LS-C60680) was used in Blocking Buffer for Fluorescent Western Blot (MB-070) for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc 4000MP Imaging System.


Requested From: 
Date Requested: 6/25/2018

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