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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GDF15 Antibody (HRP) LS‑C154107
Antibody
GDF15 antibody LS-C154107 is an HRP-conjugated rabbit polyclonal antibody to GDF15 from human and mouse. Validated for ELISA and WB.
Catalog
Size
Price
LS-C154107-100
100 µg
Unavailable

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Product Description

GDF15 antibody LS-C154107 is an HRP-conjugated rabbit polyclonal antibody to GDF15 from human and mouse. Validated for ELISA and WB.
About GDF15
Growth differentiation factor 15 (GDF15) is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. GDF15 is also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB. GDF15 mRNA is most abundant in the liver, with lower levels seen in some other tissues. Q99988 NM_004864 NP_004855.2

GDF15 Antibody, MIC1 Antibody, MIC-1 Antibody, NAG-1 Antibody, NRG-1 Antibody, NSAID-regulated gene 1 protein Antibody, PLAB Antibody, NSAID-activated gene 1 protein Antibody, Placental TGF-beta Antibody, PTGFB Antibody, GDF-15 Antibody, PTGF-beta Antibody


Specifications

Target
Human GDF15
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Purified
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
GDF15 antibody was raised against this Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E. coli corresponding to mature human NAG-1 protein. Cross reactivity to MBP was removed via cross-adsorption chromatography.
Specificity
This antibody reacts with endogenous NAG-1 protein from human tissues. A BLAST analysis using the immunizing recombinant protein suggests reactivity with NAG-1 from human and chimpanzee based on 100% and 98% homology, respectively, and with macaque based on an 94% homology, and with mouse and rat based on 70% homology with the immunizing sequence. Cross-reactivity with NAG-1 from other sources has not been determined.
Usage
This Protein-A purified, MBP-cross-adsorbed antibody is suitable for ELISA and western blotting of human and mouse NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 C-terminal specific antibodies. This antibody is useful in dual antibody immunometric assays (EIA). Specific conditions for reactivity should be optimized by the end user. Expect bands in western blots of approximately 14 kD in size corresponding to NAG-1 monomer using the appropriate cell lysate or extract. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% Gentamicin sulfate
Reconstitution
100 µl deionized water
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/22/2018

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