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Anti-GBE1 Antibody (clone 3B4) LS-C114876

Ordering

Wt. Vol. Conc. Price
- 100 µl - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GBE1 Antibodies

Anti-GBE1 Antibody (aa527-556, Biotin) LS-C222598
Rabbit Polyclonal (IgG) to Human GBE1
Human, Mouse
ICC, Immunofluorescence, Western blot, ELISA
Biotin Conjugated
Anti-GBE1 Antibody (aa527-556, FITC) LS-C222599
Rabbit Polyclonal (IgG) to Human GBE1
Human, Mouse
ICC, Immunofluorescence, Western blot, ELISA
FITC Conjugated
Anti-GBE1 Antibody (aa527-556, PE) LS-C222601
Rabbit Polyclonal (IgG) to Human GBE1
Human, Mouse
ICC, Immunofluorescence, Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 3B4] (IgG2a) to Human GBE1
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human GBE1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [3B4]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Western blot (1:2000)
  • Immunoprecipitation

Specificity and Use

GBE1 antibody was raised against full length human recombinant protein of human GBE1 (NP_000149) produced in HEK293T cell.
Human GBE1
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About GBE1

Q04446 NM_000158 NP_000149.3

GBE1 Antibody, APBD Antibody, Brancher enzyme Antibody, GBE Antibody, Glycogen-branching enzyme Antibody, GSD4 Antibody, Glycogen branching enzyme Antibody

GBE1 is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Carcinoma of prostate tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded pancreas tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded liver tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Carcinoma of liver tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded Kidney tissue using anti-GBE1 mouse monoclonal antibody. (Dilution 1:50).

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBE1.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation(IP) of GBE1 by using monoclonal anti-GBE1 antibodies (Negative control: IP without adding anti-GBE1 antibody.). For each experiment, 500ul of DDK tagged GBE1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-GBE1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
 

Requested From: United States
Date Requested: 12/2/2016

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