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Anti-FOXP3 Antibody (C-Terminus, DY550) LS-C141135

Ordering

Wt. Vol. Conc. Price
- 100 µl - $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular FOXP3 Antibodies

Anti-FOXP3 Antibody (aa100-149) LS-C31026
Rabbit Polyclonal to Human FOXP3
Human, Monkey, Rat, Bovine, Pig, Sheep
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-FOXP3 Antibody LS-C312052
Rabbit Polyclonal (IgG) to Human FOXP3
Human
IHC - Paraffin, IHC - Frozen
Unconjugated
Immunohistochemistry Image
Anti-FOXP3 Antibody (aa101-431) LS-C357445
Rabbit Polyclonal (IgG) to Human FOXP3
Human, Mouse, Rat
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Mouse FOXP3
Mouse
ICC, Immunofluorescence, Western blot, Flow Cytometry (applications tested for the base form of this product only)
DY550 Conjugated

Details

Mouse FOXP3
Rabbit
Mouse (tested or 100% immunogen sequence identity)
Polyclonal
DyLight 550. Also available Unconjugated or conjugated with Biotin, DY650, DY488.
Protein G purified
Unmodified

Applications

  • ICC
  • Immunofluorescence
  • Western blot
  • Flow Cytometry
  • (applications tested for the base form of this product only)

Specificity and Use

FOXP3 antibody was raised against a fusion protein, GST-mouse Foxp3-Flag. Mouse Foxp3 Gly43 to His100 peptide and Flag Tag were fused at C-terminal of GST protein
C-Terminus
FOXP3.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

50 mM sodium borate
Store at 4°C. Do not freeze.
For research use only.

About FOXP3

Q9BZS1 NM_014009 NP_054728.2

FOXP3 Antibody, AIID Antibody, DIETER Antibody, Forkhead box protein P3 Antibody, JM2 Antibody, Forkhead box P3 Antibody, FOXP3delta7 Antibody, XPID Antibody, PIDX Antibody, SCURFIN Antibody

FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

Immunofluorescence

Immunofluorescence
The same transfectants were stained with anti-Foxp3 antibody and HRPO-conjugated (upper panel) or Cy3-conjugated (lower panels) secondary antibody. Representative images (x100) are shown. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Jurkat Tag cells were transfected with the expression vector (vector) or with the Foxp3 expression construct (Foxp3). 24 hours after transfection, cells were lysed and analyzed by Western blot with anti-Foxp3 antiserum (1:2000 dilution). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Detection of immunoprecipitated FOXP3 from HA-tagged FOXP3 transfected Jurkat T antigen cells. Immunoprecipitated . This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow Cytometry
Transfectants from A were permeabilized and stained with anti-Foxp3 antibody and FITC-conjugated secondary antibody. Cells were analyzed by flow cytometry. Percentage of positively stained cells are shown. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/4/2016

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