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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FGR Antibody (aa517‑529) LS‑C54837
Note: This antibody replaces LS-C66022, LS-C15178
FGR antibody LS-C54837 is an unconjugated goat polyclonal antibody to FGR from human and mouse. Validated for WB.
Catalog
Size
Price
LS-C54837-100
100 µg
Unavailable

Popular FGR Products

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Anti-FGR antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4106 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml]
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

FGR antibody LS-C54837 is an unconjugated goat polyclonal antibody to FGR from human and mouse. Validated for WB.
About FGR
Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors devoid of kinase activity and contributes to the regulation of immune responses, including neutrophil, monocyte, macrophage and mast cell functions, cytoskeleton remodeling in response to extracellular stimuli, phagocytosis, cell adhesion and migration. Promotes mast cell degranulation, release of inflammatory cytokines and IgE-mediated anaphylaxis. P09769 NM_005248 NP_005239.1

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Specifications

Target
Human FGR
Host
Goat
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Monkey, Rat, Bat, Dog, Hamster, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 2 µg/ml)
Immunogen
FGR antibody was raised against synthetic peptide C-TSAEPQYQPGDQT from the C-terminus of human FGR (NP_001036194.1; NP_001036212.1; NP_005239.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Horse (92%); Bovine, Rabbit (85%).
Blocking Peptide
LS-E25255 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C54837. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa517-529
Specificity
Human FGR. This antibody is expected to recognise all three reported isoforms (NP_001036194.1; NP_001036212.1; NP_005239.1).
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Staining (0.5?g/ml) of Mouse Spleen extracts (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Staining (0.5?g/ml) of Mouse Spleen extracts (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot

Antibody staining (0.5 ug/ml) of Mouse Spleen extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.5 ug/ml) of Mouse Spleen extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Western blot

Staining (0.5?g/ml) of Mouse Spleen extracts (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Staining (0.5?g/ml) of Mouse Spleen extracts (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot

Antibody staining (0.5 ug/ml) of Mouse Spleen extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.5 ug/ml) of Mouse Spleen extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Western blot

Staining (0.5?g/ml) of Mouse Spleen extracts (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Staining (0.5?g/ml) of Mouse Spleen extracts (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot

Antibody staining (0.5 ug/ml) of Mouse Spleen extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.5 ug/ml) of Mouse Spleen extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Western blot

Staining (0.5?g/ml) of Mouse Spleen extracts (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Staining (0.5?g/ml) of Mouse Spleen extracts (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot

Antibody staining (0.5 ug/ml) of Mouse Spleen extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (0.5 ug/ml) of Mouse Spleen extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.


Requested From: 
Date Requested: 6/22/2018

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