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Catalog Number Size Price
LS-B12760-50 50 µl (1 mg/ml) $460 
FEN1 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
FEN1 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
FEN1 Antibody - Detection of human Fen1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Fen1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
FEN1 Antibody - Detection of Human Fen1 by Western Blot. Samples: Whole cell lysate (20 ug) from HeLa cells separated on a 4-12% PAGE gel. Antibodies: Affinity purified rabbit anti-Fen1 used at the indicated concentrations. Secondary antibody was goat anti-Rabbit HRP conjugate used at 1:40000 (for 0.015 ug/ml anti-Fen1) or 1:60000 (for 1 and 0.1 ug/ml anti-Fen1). Detection: ECL with the indicated exposure.
FEN1 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
FEN1 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
FEN1 Antibody - Detection of human Fen1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Fen1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
FEN1 Antibody - Detection of Human Fen1 by Western Blot. Samples: Whole cell lysate (20 ug) from HeLa cells separated on a 4-12% PAGE gel. Antibodies: Affinity purified rabbit anti-Fen1 used at the indicated concentrations. Secondary antibody was goat anti-Rabbit HRP conjugate used at 1:40000 (for 0.015 ug/ml anti-Fen1) or 1:60000 (for 1 and 0.1 ug/ml anti-Fen1). Detection: ECL with the indicated exposure.
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IHC‑plus™ Polyclonal Rabbit anti‑Human FEN1 Antibody (aa250‑300, IHC, WB) LS‑B12760

IHC‑plus™ Polyclonal Rabbit anti‑Human FEN1 Antibody (aa250‑300, IHC, WB) LS‑B12760

Note: This antibody replaces LS-C287167
Antibody:
FEN1 Rabbit anti-Human Polyclonal (aa250-300) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B12760-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
FEN1 Rabbit anti-Human Polyclonal (aa250-300) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
FEN1 antibody LS-B12760 is an unconjugated rabbit polyclonal antibody to human FEN1 (aa250-300). Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human FEN1
Synonyms
FEN1 | DNase IV | FEN-1 | HFEN-1 | Flap endonuclease 1 | MF1 | RAD2 | Maturation factor 1 | Maturation factor-1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Bovine, Sheep (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 2237 corresponding to amino acids between 250 and 300 of UniprotID: P39748
Epitope
aa250-300
Specificity
Region between residues 250 and 300 of human Flap Structure Endonuclease 1 using the numbering given in entry NP_004102.1 (GeneID 2237).
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (2 - 10 µg/mg lysate)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. 16-18 hour incubation is recommended.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FEN1
P39748 NM_004111 NP_004102.1

Validation

FEN1 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

FEN1 Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ FEN1 Antibody (aa250-300) for IHC, WB/Western, IP LS-B12760 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024