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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ER Alpha / Estrogen Receptor Antibody (aa53-301) IHC-plus™

Note: This antibody replaces LS-C167829
Estrogen Receptor antibody LS-B10527 is an unconjugated rabbit polyclonal antibody to Estrogen Receptor (ER Alpha) from human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B10527-200
200 µl
$425
Description
Estrogen Receptor antibody LS-B10527 is an unconjugated rabbit polyclonal antibody to Estrogen Receptor (ER Alpha) from human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ER Alpha / Estrogen Receptor
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Epitope
aa53-301
Specificity
This ESR1 antibody is generated from rabbits immunized with a recombinant protein between 53-301 amino acids from human ESR1.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About ER Alpha / Estrogen Receptor
P03372 NM_000125 NP_000116.2

Popular ER Alpha / Estrogen Receptor Products

Formalin-fixed, paraffin-embedded human breast carcinoma stained with peroxidase-conjugate and DAB chromogen. Note intense nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Formalin-fixed, paraffin-embedded human breast carcinoma stained with peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Western blot
Anti-Estrogen Receptor antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Antibody
Species: Bovine, Human, Mouse, Rat, Rabbit
Applications: Immunoprecipitation, Gel shift
Formalin-fixed, paraffin-embedded human breast carcinoma stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Pig, Rabbit, Sheep
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation

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Images

Immunohistochemistry

Anti-ER Alpha / Estrogen Receptor antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-ER Alpha / Estrogen Receptor antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Western blot

ESR1 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The ESR1 antibody detected the ESR1 protein (arrow).
ESR1 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The ESR1 antibody detected the ESR1 protein (arrow).

Immunohistochemistry

Anti-ER Alpha / Estrogen Receptor antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-ER Alpha / Estrogen Receptor antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Western blot

ESR1 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The ESR1 antibody detected the ESR1 protein (arrow).
ESR1 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The ESR1 antibody detected the ESR1 protein (arrow).

Immunohistochemistry

Anti-ER Alpha / Estrogen Receptor antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-ER Alpha / Estrogen Receptor antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Western blot

ESR1 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The ESR1 antibody detected the ESR1 protein (arrow).
ESR1 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The ESR1 antibody detected the ESR1 protein (arrow).

Immunohistochemistry

Anti-ER Alpha / Estrogen Receptor antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-ER Alpha / Estrogen Receptor antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

Western blot

ESR1 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The ESR1 antibody detected the ESR1 protein (arrow).
ESR1 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The ESR1 antibody detected the ESR1 protein (arrow).

Requested From: United States
Date Requested: 1/23/2019
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