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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF2AK2 / PKR Antibody (clone 1B9) LS‑C133408
PKR antibody LS-C133408 is an unconjugated mouse monoclonal antibody to human PKR (EIF2AK2). Validated for ELISA, IF and WB.
Catalog
Size
Price
LS-C133408-100
100 µg (0.49 mg/ml)
Unavailable

Popular EIF2AK2 / PKR Products

Antibody
Species: Human
Applications: Western blot
Anti-EIF2AK2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B563 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Anti-EIF2AK2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1352 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse
Applications: Western blot
EIF2AK2 antibody LS-C117004 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Dog, Rabbit
Applications: Western blot

Product Description

PKR antibody LS-C133408 is an unconjugated mouse monoclonal antibody to human PKR (EIF2AK2). Validated for ELISA, IF and WB.
About EIF2AK2 / PKR
IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). P19525 NM_002759 NP_002750.1

EIF2AK2 Antibody, p1/eIF-2A protein kinase Antibody, p68 kinase Antibody, PRKR Antibody, EIF-2A protein kinase 2 Antibody, p68 Antibody, Protein kinase RNA-activated Antibody, Tik Antibody


Specifications

Target
Human EIF2AK2 / PKR
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [1B9]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
Failed Applications
  • IHC - Paraffin
Immunogen
EIF2AK2 / PKR antibody was raised against eIF2AK2 (NP_002750, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human PKR
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4.
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Immunofluorescence of monoclonal antibody to EIF2AK2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to EIF2AK2 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of EIF2AK2 expression in transfected 293T cell line by EIF2AK2 monoclonal antibody (M01), clone 1B9.
Western blot of EIF2AK2 expression in transfected 293T cell line by EIF2AK2 monoclonal antibody (M01), clone 1B9.

Western blot

EIF2AK2 monoclonal antibody (M01), clone 1B9. Western blot of EIF2AK2 expression in MCF-7.
EIF2AK2 monoclonal antibody (M01), clone 1B9. Western blot of EIF2AK2 expression in MCF-7.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged EIF2AK2 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged EIF2AK2 is approximately 0.1 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to EIF2AK2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to EIF2AK2 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of EIF2AK2 expression in transfected 293T cell line by EIF2AK2 monoclonal antibody (M01), clone 1B9.
Western blot of EIF2AK2 expression in transfected 293T cell line by EIF2AK2 monoclonal antibody (M01), clone 1B9.

Western blot

EIF2AK2 monoclonal antibody (M01), clone 1B9. Western blot of EIF2AK2 expression in MCF-7.
EIF2AK2 monoclonal antibody (M01), clone 1B9. Western blot of EIF2AK2 expression in MCF-7.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged EIF2AK2 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged EIF2AK2 is approximately 0.1 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to EIF2AK2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to EIF2AK2 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of EIF2AK2 expression in transfected 293T cell line by EIF2AK2 monoclonal antibody (M01), clone 1B9.
Western blot of EIF2AK2 expression in transfected 293T cell line by EIF2AK2 monoclonal antibody (M01), clone 1B9.

Western blot

EIF2AK2 monoclonal antibody (M01), clone 1B9. Western blot of EIF2AK2 expression in MCF-7.
EIF2AK2 monoclonal antibody (M01), clone 1B9. Western blot of EIF2AK2 expression in MCF-7.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged EIF2AK2 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged EIF2AK2 is approximately 0.1 ng/ml as a capture antibody.

Immunofluorescence

Immunofluorescence of monoclonal antibody to EIF2AK2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to EIF2AK2 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot of EIF2AK2 expression in transfected 293T cell line by EIF2AK2 monoclonal antibody (M01), clone 1B9.
Western blot of EIF2AK2 expression in transfected 293T cell line by EIF2AK2 monoclonal antibody (M01), clone 1B9.

Western blot

EIF2AK2 monoclonal antibody (M01), clone 1B9. Western blot of EIF2AK2 expression in MCF-7.
EIF2AK2 monoclonal antibody (M01), clone 1B9. Western blot of EIF2AK2 expression in MCF-7.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged EIF2AK2 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged EIF2AK2 is approximately 0.1 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/20/2018

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