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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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EIF2AK2 / PKR Antibody (phospho-Thr446) LS-C17480

PKR antibody LS-C17480 is an unconjugated rabbit polyclonal antibody to human PKR (EIF2AK2). Validated for WB.
Catalog
Size
Price
LS-C17480-100
100 µg
$680
Description
PKR antibody LS-C17480 is an unconjugated rabbit polyclonal antibody to human PKR (EIF2AK2). Validated for WB.
Target
Human EIF2AK2 / PKR
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 2 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
EIF2AK2 / PKR antibody was raised against peptide corresponding to amino acids surrounding Thr446 of human PKR.
Epitope
pThr446
Specificity
Recognizes human PKR phosphorylated at Thr446, Mr 68kD. A non-specific protein was also detected, Mr ~75kD, which is inhibited by the immunizing peptide.
Usage
Suitable for use in Western Blot. Western Blot: 0.5-2.0 ug/ml detects phospho-PKR in RIPA lysates from HeLa cells that had. been stimulated with IFN alpha overnight followed by treatment with calyculin A for 15 minutes. Beadlyte Peptide Specificity Assay: 1:1000-1:5000 dilutions were incubated with a cocktail of. microspheres conjugated to either the phosphorylated or non phosphorylated peptides. Treated HeLa (lane 1) or untreated HeLa (lane2) cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-PKR (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho-PKR (68kD).
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About EIF2AK2 / PKR
P19525 NM_002759 NP_002750.1

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Requested From: United States
Date Requested: 4/21/2019
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