PKR antibody LS-C17480 is an unconjugated rabbit polyclonal antibody to human PKR (EIF2AK2). Validated for WB.
Human EIF2AK2 / PKR
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot (0.5 - 2 µg/ml)
- (applications tested for the base form of this product only)
EIF2AK2 / PKR antibody was raised against peptide corresponding to amino acids surrounding Thr446 of human PKR.
Recognizes human PKR phosphorylated at Thr446, Mr 68kD. A non-specific protein was also detected, Mr ~75kD, which is inhibited by the immunizing peptide.
Suitable for use in Western Blot. Western Blot: 0.5-2.0 ug/ml detects phospho-PKR in RIPA lysates from HeLa cells that had. been stimulated with IFN alpha overnight followed by treatment with calyculin A for 15 minutes. Beadlyte Peptide Specificity Assay: 1:1000-1:5000 dilutions were incubated with a cocktail of. microspheres conjugated to either the phosphorylated or non phosphorylated peptides. Treated HeLa (lane 1) or untreated HeLa (lane2) cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-PKR (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho-PKR (68kD).
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.