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EIF2AK2 / PKR Antibody (phospho-Thr446) LS-C17480

Antibody

EIF2AK2 / PKR Antibody (phospho-Thr446) LS-C17480

Antibody:
Rabbit Polyclonal to Human EIF2AK2 / PKR
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$680 
LS-C17480-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human EIF2AK2 / PKR
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
PKR antibody LS-C17480 is an unconjugated rabbit polyclonal antibody to human PKR (EIF2AK2). Validated for WB.

Target
Human EIF2AK2 / PKR
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 2 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
EIF2AK2 / PKR antibody was raised against peptide corresponding to amino acids surrounding Thr446 of human PKR.
Epitope
pThr446
Specificity
Recognizes human PKR phosphorylated at Thr446, Mr 68kD. A non-specific protein was also detected, Mr ~75kD, which is inhibited by the immunizing peptide.
Usage
Suitable for use in Western Blot. Western Blot: 0.5-2.0 ug/ml detects phospho-PKR in RIPA lysates from HeLa cells that had. been stimulated with IFN alpha overnight followed by treatment with calyculin A for 15 minutes. Beadlyte Peptide Specificity Assay: 1:1000-1:5000 dilutions were incubated with a cocktail of. microspheres conjugated to either the phosphorylated or non phosphorylated peptides. Treated HeLa (lane 1) or untreated HeLa (lane2) cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-PKR (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phospho-PKR (68kD).
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EIF2AK2 / PKR
P19525 NM_002759 NP_002750.1

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Species: Human
Applications: IHC, IHC - Paraffin, Flow Cytometry

Requested From: United States
Date Requested: 7/19/2019
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