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Antibodies > DYKDDDDK Tag > LS-C204263
 
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Catalog Number Size Price
LS-C204263-600 600 µg (3 mg/ml) $422 
DYKDDDDK Tag Antibody - Western blot. Anti-DYKDDDDK antibody at 1:1000 dilution. 293 cells transfected with Flag-poly Ub. Lysate at 100 ug per lane. Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

Polyclonal Goat DYKDDDDK Tag Antibody (WB) LS‑C204263

Polyclonal Goat DYKDDDDK Tag Antibody (WB) LS‑C204263

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Antibody:
DYKDDDDK Tag Goat Polyclonal Antibody
Application:
WB
Format:
Unconjugated, Unmodified
Price
Catalog Number
$422
LS-C204263-600
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DYKDDDDK Tag Goat Polyclonal Antibody
Application:
WB
Format:
Unconjugated, Unmodified

Specifications

Description
DYKDDDDK Tag antibody LS-C204263 is an unconjugated goat polyclonal antibody to DYKDDDDK Tag. Validated for WB.
Target
DYKDDDDK Tag
Host
Goat
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Purified recombinant peptide DYKDDDDK produced in E. coli.
Specificity
This antibody recognizes recombinant proteins containing DYKDDDDK epitope tag fused to either amino- or carboxy-terminal of targeted proteins in transfected cells.
Applications
  • Western blot (1:500 - 1:2000)
Presentation
PBS, 0.05% Sodium Azide, 20% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

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Customer Reviews (0)

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Species:
Applications: IHC, Western blot, ELISA
DYKDDDDK Tag Antibody - Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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DYKDDDDK Tag Antibody - Antibody for the detection of FLAG conjugated proteins - Western Blot. Affinity Purified Antibody to detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins by western blot. This antibody was used at a dilution of 1:2500 to detect 1.0 ug of recombinant protein containing either the FLAG epitope tag linked at the carboxy (C) or the amino (N) terminus of the recombinant protein. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
DYKDDDDK Tag Antibody LS‑C154223
Species:
Applications: IHC, Western blot, ELISA
See all DYKDDDDK Tag Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 9/18/2024
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