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Anti-DYKDDDDK Tag Antibody LS-C203597

Ordering

Wt. Vol. Conc. Price
100 µg - - $275
200 µg - - $375
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

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Mouse Monoclonal (IgG2b,k)
Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

DYKDDDDK Tag
Mouse
IgG2b,k Monoclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence
  • Western blot
  • Immunoprecipitation (1 µg/ml)
  • ELISA (0.05 - 0.2 µg/ml)

Specificity and Use

DYKDDDDK Tag antibody was raised against a synthetic peptide (DYKDDDDK) coupled to KLH
C-terminal, N-terminal and internal tagged fusion proteins.

Packaging

Lyophilized from PBS, pH 7.4, 0.02% sodium azide.
Distilled water
Store lyophilized at -20°C. The reconstituted product can be stored for short term at 4 °C or long term at -20 °C or below. Avoid freeze/thaw cycles.
For research use only.

About DYKDDDDK Tag


Immunofluorescence

Immunofluorescence
293 cells transfected with C-terminal DYKDDDDK tag protein. Primary antibody: 1 ?g/ml THETM Anti-DYKDDDDK-tag Monoclonal Antibody (Mouse). Secondary antibody: 2 ?g/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121).

Immunofluorescence

Immunofluorescence
293 cells transfected with N-terminal DYKDDDDK tag protein. Primary antibody: 1 ug/ml THETM Anti-DYKDDDDK-tag Monoclonal Antibody (Mouse). Secondary antibody: 2 ug/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121).

Western blot

Western blot
Consistency analysis of Batch 1# and 2# of THETM DYKDDDDK Tag Antibody, mAb, Mouse (1 ug/ml) by Western blot, showing that signal remains consistent from Lot to Lot. The assay was performed with DYKDDDDK-tagged fusion protein. The signal was developed with IRDye 800 Conjugated Goat Anti-Mouse IgG.

Western blot

Western blot
Western blot of E. coli lysate containing DYKDDDDK-tagged fusion protein and its immunoprecipitates. 1: E. coli lysate containing DYKDDDDK-tagged fusion protein. 2: Immunoprecipitates of free E. coli lysate incubated with DYKDDDDK Tag Antibody, mAb, Mouse (2 ug/ml) and Protein A. 3: Immunoprecipitates of E. coli lysate containing DYKDDDDK-tagged fusion protein incubated with DYKDDDDK Tag Antibody, mAb, Mouse (2 ug/ml) and Protein A.

Western blot

Western blot
Comparison of DYKDDDDK Tag Antibody, mAb, Mouse (A: 1 ug/ml) with Mouse Anti- DYKDDDDK Tag mAb (B: Company S, clone M2, 1 ug/ml) by Western blot. Lane 1 N-terminal DYKDDDDK-tagged fusion protein. Lane 2 Internal DYKDDDDK-tagged fusion protein. Lane 3 C-terminal DYKDDDDK-tagged fusion protein. Predicted Size: N-terminal DYKDDDDK-tagged fusion protein 52 kD. Internal DYKDDDDK-tagged fusion protein 55 kD. C-terminal DYKDDDDK-tagged fusion protein 55 kD. Observed Size: N-terminal DYKDDDDK-tagged fusion protein 52 kD. Internal DYKDDDDK-tagged fusion protein 55 kD. C-terminal DYKDDDDK-tagged fusion protein 55 kD. The signal was developed with IRDye 800 Conjugated Goat Anti-Mouse IgG.

Western blot

Western blot
Loading: Lane 1-3 Multiple Tag (Purified) (400 ng, 80 ng,16 ng). Lane 4. 293 cell lysate 10 ul. Primary antibody: A. 1 ug/ml THETM Mouse Anti-DYKDDDDK-tag Monoclonal Antibody). B. 1 ug/ml Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (Company S, clone M2). Secondary antibody: IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG (ROCKLAND, 1: 10000,610-132-121).

Requested From: United States
Date Requested: 12/7/2016

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