DYKDDDDK Tag

Mouse

IgG2a,k Monoclonal

29E4.G7

Unconjugated

Protein A affinity chromatography

Unmodified

This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the termini to facilitate coupling.

This monoclonal anti-FLAG™ tag antibody detects over-expressed proteins containing the FLAG™ epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.

• IHC (1:1000 - 1:5000)
• Western blot (1:2000 - 1:10000)
• Flow Cytometry
• ELISA (1:150000 - 1:250000)

This antibody is optimally suited for monitoring the expression of DYKDDDDK tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the DYKDDDDK epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.