Target
Rat DRD2 / Dopamine Receptor D2
Synonyms
DRD2 | D2R | D(2) dopamine receptor | D2DR
Reactivity
Rat, Dog
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
A 27 amino acid peptide sequence from the rat D2 long form within the cytoplasmic loop #3. No significant homology with other receptors (D1, D2 short, and D3-D5).
Epitope
3rd Cytoplasmic Domain
Specificity
Recognizes rat Dopamine Receptor D2 long form. Recognizes canine D2R. Species sequence Homology: Human, bovine, and canine: 96%, Xenopus: 64%.
Applications
- Western blot (1 - 10 µg/ml)
- ELISA (0.5 - 1 µg/ml)
Usage
Suitable for use in Western Blot and ELISA. Western Blot: 1-10 ug/ml (Chemiluminescence technique). The predicted size of D2L form is approximately 50kD. However, additional bands at approximately 60 and 100kD, and others are typically seen in various tissues. Approximately 100-400 ug of membrane protein/lane should be loaded to visualize various bands. ELISA: 0.5-1.0 ug/ml (1 ug/ml immunogen peptide/well).
Presentation
100 mM Tris, pH 7.5, 0.05% Sodium Azide, 40% Glycerol, 0.2% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DRD2 / Dopamine Receptor D2