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Anti-DEFA5 / Defensin 5 Antibody (clone 8C8) IHC-plus™ LS-B2904

Note: This antibody replaces LS-C49165

Ordering

Wt. Vol. Conc. Price
- 100 µl 2.35 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular DEFA5 / Defensin 5 Antibodies

Anti-DEFA5 / Defensin 5 Antibody LS-C50934
Mouse Monoclonal (IgG2a) to Rabbit DEFA5 / Defensin 5
Rabbit
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry
Unconjugated
Anti-DEFA5 / Defensin 5 Antibody (clone 8C8) IHC-plus™ LS-B2904
Mouse Monoclonal [clone 8C8] to Human DEFA5 / Defensin 5
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-DEFA5 / Defensin 5 Antibody IHC-plus™ LS-B3145
Mouse Monoclonal (IgG2b) to Human DEFA5 / Defensin 5
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 8C8] to Human DEFA5 / Defensin 5
Human
IHC - Paraffin, Immunofluorescence, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human DEFA5 / Defensin 5
Mouse
Human (tested or 100% immunogen sequence identity)
Monoclonal [8C8]
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

DEFA5 / Defensin 5 antibody was raised against full-length proHD5 protein. [Swiss-Prot# Q01523] The propeptide is the full-length protein. In the mature form, it is cleaved at residue 63 (alanine)
HD5. It does not cross-react with HD6, lysozyme or sPLA2.
Immunohistochemistry: LS-B2904 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2904 was determined to be 10 ug/ml.

Packaging

Tris-glycine, 150 mM sodium chloride, 0.05% sodium azide.
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
For research use only.

About DEFA5 / Defensin 5

Q01523 NM_021010 NP_066290.1

DEFA5 Antibody, DEF5 Antibody, Defensin, alpha 5 Antibody, Defensin 5 Antibody, Defensin-5 Antibody, HD-5 Antibody, HD5(20-94) Antibody

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8.

Immunohistochemistry

Immunohistochemistry
Anti-DEFA5 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2904 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/3/2016

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