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Anti-DDB1 Antibody (Internal) IHC-plus™ LS-B364

Note: This antibody replaces LS-C18964

Ordering

Wt. Vol. Conc. Price
- 50 µl 85 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular DDB1 Antibodies

Anti-DDB1 Antibody (Internal) IHC-plus™ LS-B364
Rabbit Polyclonal to Human DDB1
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-DDB1 Antibody (C-Terminus) IHC-plus™ LS-B1692
Rabbit Polyclonal (IgG) to Human DDB1
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-DDB1 Antibody IHC-plus™ LS-B3138
Mouse Monoclonal (IgG1,k) to Human DDB1
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (2)
Rabbit Polyclonal to Human DDB1
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human DDB1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Delipidated and defibrinated
Unmodified

Applications

  • IHC - Paraffin (1:500)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • ELISA (1:2000 - 1:10000)

Specificity and Use

DDB1 antibody was raised against synthetic peptide from human DDB1.
Internal
Amino acids 198-213 of Human DDB1 (internal) coupled to KLH.
Immunohistochemistry: LS-B364 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B364 was determined to be 1:500.

Packaging

0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (2)

1
The naturally occurring mutants of DDB are impaired in stimulating nuclear import of the p125 subunit and E2F1-activated transcription. Shiyanov P, Hayes SA, Donepudi M, Nichols AF, Linn S, Slagle BL, Raychaudhuri P. Molecular and cellular biology. 1999 19:4935-43. [PubMed:10373543] [PMC:PMC84302]
2
Cullin 4A associates with the UV-damaged DNA-binding protein DDB. Shiyanov P, Nag A, Raychaudhuri P. The Journal of biological chemistry. 1999 274:35309-12. [PubMed:10585395]

About DDB1

Q16531 NM_001923 NP_001914.3

DDB1 Antibody, DDB p127 subunit Antibody, DDBA Antibody, HBV X-associated protein 1 Antibody, UV-DDB1 Antibody, XAP-1 Antibody, XPE Antibody, XPE-binding factor Antibody, UV-damaged DNA-binding factor Antibody, XPCE Antibody, DNA damage-binding protein 1 Antibody, DNA damage-binding protein a Antibody, UV-DDB 1 Antibody, XAP1 Antibody, XPE-BF Antibody

Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches.

Immunohistochemistry

Immunohistochemistry
Anti-DDB1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B364 dilution 1:500.
 

Requested From: United States
Date Requested: 12/5/2016

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