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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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DDB1

damage-specific DNA binding protein 1, 127kDa

Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.

Gene Name: damage-specific DNA binding protein 1, 127kDa
Synonyms: DDB1, DDB p127 subunit, DDBA, HBV X-associated protein 1, UV-DDB1, XAP-1, XPE, XPE-binding factor, UV-damaged DNA-binding factor, XPCE, DNA damage-binding protein 1, DNA damage-binding protein a, UV-DDB 1, XAP1, XPE-BF
Target Sequences: NM_001923 NP_001914.3 Q16531

Publications (2)

1
The naturally occurring mutants of DDB are impaired in stimulating nuclear import of the p125 subunit and E2F1-activated transcription. Shiyanov P, Hayes SA, Donepudi M, Nichols AF, Linn S, Slagle BL, Raychaudhuri P. Molecular and cellular biology. 1999 19:4935-43. [PubMed:10373543] [PMC:PMC84302] Related Antibodies: LS-B364.
2
Cullin 4A associates with the UV-damaged DNA-binding protein DDB. Shiyanov P, Nag A, Raychaudhuri P. The Journal of biological chemistry. 1999 274:35309-12. [PubMed:10585395] Related Antibodies: LS-B364.

Filtered By: None
Target
DDB1 (41)
Reactivity
Human (40)
Mouse (19)
Rat (13)
Bat (1)
Bovine (4)
Cat (1)
Dog (4)
Hamster (1)
Horse (4)
Monkey (2)
Pig (2)
Primate (1)
Rabbit (3)
Xenopus (3)
Application
IHC (19)
IHC-P (13)
WB (36)
Flo (1)
ELISA (15)
ICC (1)
IF (2)
IP (10)
Peptide-ELISA (1)
PLA (1)
Host
rabbit (22)
mouse (12)
goat (7)
Isotype
IgG (20)
IgG1,k (7)
IgG2a (1)
IgG2a,k (1)
Clonality
monoclonal mc (12)
polyclonal pc (29)
Clone
1322 (1)
4C5 (1)
Format
AP Conjugated (2)
APC Conjugated (1)
Biotin Conjugated (4)
FITC Conjugated (4)
HRP Conjugated (4)
PE Conjugated (2)
Unconjugated (24)
Epitope
Internal (5)
C-Terminus (4)
aa1011-1140 (1)
aa1128-1140 (1)
aa385-435 (1)
aa749-1140 (1)
Publications
No (40)
Yes (1)
Showing 1-25 of 41 results

DDB1 Antibody (Internal) IHC-plus™ LS-B364
Publications (2)
Publications (2)
Rabbit Polyclonal to Human DDB1
Human
ELISA IHC IHC-P IP WB
Unconjugated
50µl 85mg/ml  $425.00
Anti-DDB1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-DDB1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

DDB1 Antibody IHC-plus™ LS-B3138
Mouse Monoclonal (IgG1,k) to Human DDB1
Human
ELISA IHC IHC-P IP WB
Unconjugated
50µg 0.5mg/ml  $485.00
Anti-DDB1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-DDB1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-DDB1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-DDB1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

DDB1 Antibody (HRP) LS-C545575
Mouse Monoclonal (IgG1,k) to Human DDB1
Human
ELISA IP WB
HRP Conjugated
100µl  $800.00

DDB1 Antibody (Biotin) LS-C525455
Mouse Monoclonal (IgG1,k) to Human DDB1
Human
ELISA IP WB
Biotin Conjugated
100µl  $800.00

DDB1 Antibody (FITC) LS-C535470
Mouse Monoclonal (IgG1,k) to Human DDB1
Human
ELISA IP WB
FITC Conjugated
100µl  $800.00

DDB1 Antibody (APC) LS-C516346
Mouse Monoclonal (IgG1,k) to Human DDB1
Human
ELISA IP WB
APC Conjugated
100µl  $800.00

DDB1 Antibody (AP) LS-C507488
Mouse Monoclonal (IgG1,k) to Human DDB1
Human
ELISA IP WB
AP Conjugated
100µl  $800.00

DDB1 Antibody (C-Terminus) IHC-plus™ LS-B1692
Rabbit Polyclonal (IgG) to Human DDB1
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-DDB1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-DDB1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

DDB1 Antibody (clone 1322) IHC-plus™ LS-B1691
Mouse Monoclonal [clone 1322] (IgG) to Human DDB1
Human
ELISA IHC IHC-P WB
Unconjugated
50µl 3mg/ml  $425.00
Anti-DDB1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-DDB1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

DDB1 Antibody (aa1128-1140) IHC-plus™ LS-B11164
Goat Polyclonal to Human DDB1
Xenopus, Bat, Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Human, Monkey
IHC IHC-P Peptide-ELISA WB
Unconjugated
50µg 0.5mg/ml  $425.00
Anti-DDB1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-DDB1 Antibody(1 µg/ml) staining of NSO lysate (1E5 cells per lane). Detected using chemiluminescence.
Anti-DDB1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-DDB1 Antibody(1 µg/ml) staining of NSO lysate (1E5 cells per lane). Detected using chemiluminescence.

DDB1 Antibody (aa749-1140) LS-C185341
Rabbit Polyclonal (IgG) to Human DDB1
Human
ICC IHC IHC-P IP WB
Unconjugated
100µl 0.81mg/ml  $335.00
IHC of paraffin-embedded Cal27 Xenograft using DDB1 antibody at 1:500 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using DDB1 antibody at 1:200 dilution.
IHC of paraffin-embedded Cal27 Xenograft using DDB1 antibody at 1:500 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using DDB1 antibody at 1:200 dilution.

DDB1 Antibody (C-Terminus) LS-C287347
Rabbit Polyclonal (IgG) to Human DDB1
Xenopus, Rat, Horse, Rabbit, Mouse, Dog, Bovine, Human
IF IHC IHC-P IP PLA WB
Unconjugated
100µl 0.2mg/ml  $395.00
Detection of Human and Mouse DDB1 by Immunohistochemistry. Sample: FFPE section of human pancreatic islet cell tumor (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDB1 used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.
Detection of Human and Mouse DDB1 by Immunohistochemistry. Sample: FFPE section of human pancreatic islet cell tumor (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDB1 used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB.

DDB1 Antibody (clone 4C5) LS-C133877
Mouse Monoclonal [clone 4C5] (IgG2a,k) to Human DDB1
Human
ELISA IHC IHC-P WB
Unconjugated
100µg  $355.00
Immunoperoxidase of monoclonal antibody to DDB1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged DDB1 is approximately 0.3 ng/ml as a capture antibody.
Immunoperoxidase of monoclonal antibody to DDB1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 3 ug/ml]
Detection limit for recombinant GST tagged DDB1 is approximately 0.3 ng/ml as a capture antibody.

DDB1 Antibody LS-C751732
Mouse Monoclonal (IgG2a) to Human DDB1
Human
ELISA IHC WB
Unconjugated
100µg  $355.00

DDB1 Antibody (aa1011-1140) LS-C407791
Rabbit Polyclonal to Human DDB1
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
100µg  $355.00
DDB1 antibody IHC-paraffin. IHC(P): Mouse Liver Tissue.
DDB1 antibody IHC-paraffin. IHC(P): Rat Liver Tissue.
DDB1 antibody IHC-paraffin. IHC(P): Mouse Liver Tissue.
DDB1 antibody IHC-paraffin. IHC(P): Rat Liver Tissue.

DDB1 Antibody LS-C332277
Rabbit Polyclonal (IgG) to Human DDB1
Mouse, Rat, Human
IHC WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded human cervical cancer tissue.
Immunohistochemistry of paraffin-embedded human metrocarcinoma tissue.
Immunohistochemistry of paraffin-embedded human cervical cancer tissue.
Immunohistochemistry of paraffin-embedded human metrocarcinoma tissue.

DDB1 Antibody LS-C490253
Rabbit Polyclonal (IgG) to Human DDB1
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
100µg  $365.00
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image

DDB1 Antibody LS-C751731
Rabbit Polyclonal (IgG) to Human DDB1
Human
ELISA IHC WB
Unconjugated
100µg  $335.00

DDB1 Antibody (C-Terminus) LS-C286404
Rabbit Polyclonal (IgG) to Human DDB1
Xenopus, Rat, Horse, Rabbit, Mouse, Dog, Bovine, Human
IF IHC IHC-P
Unconjugated
100µl  $415.00
Detection of Human DDB1 by Immunohistochemistry. Sample: FFPE section of human breast tumor. Antibody: Affinity purified rabbit anti-DDB1 used at a dilution of 1:250.
Detection of Mouse DDB1 by Immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma tumor. Antibody: Affinity purified rabbit anti-DDB1 used at a dilution of 1:250.
Detection of Human DDB1 by Immunohistochemistry. Sample: FFPE section of human breast tumor. Antibody: Affinity purified rabbit anti-DDB1 used at a dilution of 1:250.
Detection of Mouse DDB1 by Immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma tumor. Antibody: Affinity purified rabbit anti-DDB1 used at a dilution of 1:250.

DDB1 Antibody LS-C402236
Rabbit Polyclonal (IgG) to Human DDB1
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human cervical cancer using DDB1 Polyclonal Antibody at dilution of 1:25.
Western blot analysis of Human fetal small intestine, liver cancer and Lymphoma Lymphoma, 293T and A549 cell, using DDB1 Polyclonal Antibody at dilution of 1:500.
Immunohistochemistry of Human cervical cancer using DDB1 Polyclonal Antibody at dilution of 1:25.
Western blot analysis of Human fetal small intestine, liver cancer and Lymphoma Lymphoma, 293T and A549 cell, using DDB1 Polyclonal Antibody at dilution of 1:500.

DDB1 Antibody LS-C405529
Rabbit Polyclonal (IgG) to Human DDB1
Mouse, Rat, Human
ELISA IHC WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human cervical cancer using DDB1 Polyclonal Antibody at dilution of 1:5.
Western blot analysis of MCF-7, HeLa and HepG2 cell, using DDB1 Polyclonal Antibody at dilution of 1:350.
Immunohistochemistry of Human cervical cancer using DDB1 Polyclonal Antibody at dilution of 1:5.
Western blot analysis of MCF-7, HeLa and HepG2 cell, using DDB1 Polyclonal Antibody at dilution of 1:350.

DDB1 Antibody LS-C671873
Rabbit Polyclonal (IgG) to Human DDB1
Human
ELISA IHC IHC-P
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry image of paraffin-embedded human kidney tissue at a dilution of 1:100
Immunohistochemistry image of paraffin-embedded human kidney tissue at a dilution of 1:100

DDB1 Antibody LS-C482385
Rabbit Polyclonal to Human DDB1
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
50µl 1mg/ml  $255.00; 100µl 1mg/ml  $295.00; 200µl 1mg/ml  $375.00
Immunohistochemical analysis of DDB1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of DDB1 expression in A549 (A); mouse brain (B); mouse spleen (C); mouse liver (D) whole cell lysates.
Immunohistochemical analysis of DDB1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Western blot analysis of DDB1 expression in A549 (A); mouse brain (B); mouse spleen (C); mouse liver (D) whole cell lysates.

DDB1 Antibody LS-C341152
Rabbit Polyclonal to Human DDB1
Mouse, Rat, Human
IHC WB
Unconjugated
100µl 1mg/ml  $385.00

DDB1 Antibody (aa385-435) LS-C359424
Rabbit Polyclonal to Human DDB1
Mouse, Dog, Bovine, Rat, Pig, Cat, Horse, Primate, Human
Flo WB
Unconjugated
100µg 0.5mg/ml  $335.00
Western Blot: DDB1 Antibody - Analysis of DDB1 using DDB1 antibody. Human liver tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) HeLa and 4) Daudi lysate probed with 2 ug/ml of DDB1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Flow Cytometry: DDB1 Antibody - Intracellular analysis of DDB1 in human HeLa and mouse RAW cells using DDB1 antibody at 0.2 ug/10^6 cells. Red represents cells with DDB1 antibody, green represents isotype control.
Western Blot: DDB1 Antibody - Analysis of DDB1 using DDB1 antibody. Human liver tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) HeLa and 4) Daudi lysate probed with 2 ug/ml of DDB1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Flow Cytometry: DDB1 Antibody - Intracellular analysis of DDB1 in human HeLa and mouse RAW cells using DDB1 antibody at 0.2 ug/10^6 cells. Red represents cells with DDB1 antibody, green represents isotype control.
Showing 1-25 of 41 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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