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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Collagen IV Antibody (clone 1042) LS‑C121651
Antibody
Collagen IV antibody LS-C121651 is an unconjugated mouse monoclonal antibody to human Collagen IV. Validated for IHC and WB.
Catalog
Size
Price
LS-C121651-1000
1000 µl
Unavailable

Popular Collagen IV Products

Anti-Collagen IV antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2717 concentration 2.5 ug/ml.
Species: Human, Bovine
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Anti-Collagen IV antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3654 dilution 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, ELISA
Antibody
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, ELISA
Anti-Collagen IV antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Mouse, Rat, Cat, Dog, Pig, Fish
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Human, Kidney, glomerulus: Formalin-Fixed Paraffin-Embedded (FFPE)
Species: Human, Rat, Bovine, Pig
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot

Product Description

Collagen IV antibody LS-C121651 is an unconjugated mouse monoclonal antibody to human Collagen IV. Validated for IHC and WB.
About Collagen IV
Type IV Collagen (ColIV or Col4) is a type of collagen found primarily in the basal lamina. The collagen IV C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause the collagen to form in a sheet, the form of the basal lamina.

Specifications

Target
Human Collagen IV
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal [1042]
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:20)
  • IHC - Frozen (1:10 - 1:20)
  • Western blot (1:10 - 1:20)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Collagen IV antibody was raised against collagen IV isolated from human placenta.
Specificity
Recognizes human collagen type IV. Does not cross-react with any other collagen types.
Usage
Suitable for use in Western Blot and Immunohistochemistry. Western Blot: Exclusively reactive with basal membranes. Immunohistochemistry (Frozen, paraffin): 1:10-1:20 using the indirect method. Dilute immediately before use with PBS. Fixation with acetone for 5 min. at -20°C is recommended for frozen sections. Paraffin material should be fixed with neutral formalin. For such tissue sections proteolytic pretreatment with pepsin is recommended.
Presentation
0.15 M PBS, 1% BSA, 0.02% sodium azide
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/20/2018

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