(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:10 - 1:20)
- IHC - Frozen (1:10 - 1:20)
- Western blot (1:10 - 1:20)
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Collagen IV antibody was raised against collagen IV isolated from human placenta.
Recognizes human collagen type IV. Does not cross-react with any other collagen types.
Suitable for use in Western Blot and Immunohistochemistry. Western Blot: Exclusively reactive with basal membranes. Immunohistochemistry (Frozen, paraffin): 1:10-1:20 using the indirect method. Dilute immediately before use with PBS. Fixation with acetone for 5 min. at -20°C is recommended for frozen sections. Paraffin material should be fixed with neutral formalin. For such tissue sections proteolytic pretreatment with pepsin is recommended.
0.15 M PBS, 1% BSA, 0.02% sodium azide
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
For research use only.
This antibody carries the LSBio 100% Guarantee.