Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot
Mouse Monoclonal [clone 1042] (IgG2b) to Human Collagen IV
IHC, IHC - Paraffin, IHC - Frozen, Western blot
Human Collagen IV
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal 
IHC - Paraffin (1:10 - 1:20)
IHC - Frozen (1:10 - 1:20)
Western blot (1:10 - 1:20)
Specificity and Use
Collagen IV antibody was raised against collagen IV isolated from human placenta.
Recognizes human collagen type IV. Does not cross-react with any other collagen types.
Suitable for use in Western Blot and Immunohistochemistry. Western Blot: Exclusively reactive with basal membranes. Immunohistochemistry (Frozen, paraffin): 1:10-1:20 using the indirect method. Dilute immediately before use with PBS. Fixation with acetone for 5 min. at -20°C is recommended for frozen sections. Paraffin material should be fixed with neutral formalin. For such tissue sections proteolytic pretreatment with pepsin is recommended.
0.15 M PBS, 1% BSA, 0.02% sodium azide
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months.
For research use only.
About Collagen IV
Type IV Collagen (ColIV or Col4) is a type of collagen found primarily in the basal lamina. The collagen IV C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause the collagen to form in a sheet, the form of the basal lamina.