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Anti-Collagen IV Antibody (clone 1042) LS-C121651

Catalog Size Price
LS-C121651-1000 1000 µl Unavailable

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100% Guaranteed
Mouse Monoclonal [clone 1042] (IgG2b) to Human Collagen IV
Human
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated

Details

Human Collagen IV
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [1042]
Unconjugated
Ascites
Unmodified

Applications

  • IHC - Paraffin (1:10 - 1:20)
  • IHC - Frozen (1:10 - 1:20)
  • Western blot (1:10 - 1:20)

Specificity and Use

Collagen IV antibody was raised against collagen IV isolated from human placenta.
Recognizes human collagen type IV. Does not cross-react with any other collagen types.
Suitable for use in Western Blot and Immunohistochemistry. Western Blot: Exclusively reactive with basal membranes. Immunohistochemistry (Frozen, paraffin): 1:10-1:20 using the indirect method. Dilute immediately before use with PBS. Fixation with acetone for 5 min. at -20°C is recommended for frozen sections. Paraffin material should be fixed with neutral formalin. For such tissue sections proteolytic pretreatment with pepsin is recommended.

Packaging

0.15 M PBS, 1% BSA, 0.02% sodium azide
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months.
For research use only.

About Collagen IV

Type IV Collagen (ColIV or Col4) is a type of collagen found primarily in the basal lamina. The collagen IV C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause the collagen to form in a sheet, the form of the basal lamina.

Requested From: 
Date Requested: 12/11/2017

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