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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16212-0.05 | 0.05 ml | $460 |
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PathPlus™ Monoclonal Mouse anti‑Human Collagen IV Antibody (Concentrated, IHC, WB) LS‑B16212
PathPlus™ Monoclonal Mouse anti‑Human Collagen IV Antibody (Concentrated, IHC, WB) LS‑B16212
Note: This antibody replaces LS-C757336
Antibody:
Collagen IV Mouse anti-Human Monoclonal (Concentrated) Antibody
Application:
IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Concentrated
Other formats:
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
Collagen IV Mouse anti-Human Monoclonal (Concentrated) Antibody
Application:
IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Concentrated
Other formats:
Specifications
Description
Collagen IV is a collagen present in all metazoan phyla. It is found in the basal lamina, where it takes the form of structural sheets in a crosslinked extracellular matrix. The collagen IV alpha-3 subunit (Col4A3) has been implicated as the target antigen in the autoimmune disease Goodpasture syndrome, and mutations in Collagen IV have been implicated in the hereditary Alport syndrome. In immunohistochemistry, antibodies to collagen IV react with basement membranes in a wide variety of tissues, including kidney, skin, striated and smooth muscle, spleen, lymph node, lung, placenta, and tendon.
References: Biol Cell. 1996;88(1-2):37-44, PMID: 9175266; N Engl J Med. 2003; 348:2543-2556, DOI: 10.1056/NEJMra022296; Matrix Biol. 2017 Jan;57-58:45-54, PMID: 27576055;
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Concentrated.
Also available Ready-to-Use.
Immunogen
Human placenta.
Applications
- IHC - Paraffin (1:100)
- Western blot (1:100 - 1:200)
- ELISA (1:100 - 1:200)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Dilute antibody to its recommended working dilution using the same buffer defined in the Presentation. Manual IHC Protocol: 1) Perform Heat-Induced Epitope Retrieval at pH 9 for 10 to 30 minutes. 2) Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System) 3) Dilute antibody 1:100-1:200 before applying. Incubate antibody for 10 to 30 minutes at room temperature. 4) Add secondary antibody and incubate for 20 to 30 minutes at room temperature. 5) Add DAB or Fast Red and incubate for 5 to 10 minutes at room temperature. 6) Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds. Dehydrate and apply coverslip.
Presentation
Tris, pH 7.3-7.7, 1% BSA, <0.1% Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Validation
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ Collagen IV Antibody (Concentrated) for IHC, WB/Western, ELISA LS-B16212 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Rat, Bovine, Pig
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot
Species:
Mammal, Human, Bovine
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, ELISA
Species:
Human, Rat, Bovine, Pig
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot
Species:
Human, Mouse, Rat, Cat, Dog, Pig, Fish
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Frozen, ICC, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
Date Requested: 4/27/2024
