(tested or 100% immunogen sequence identity)
- IHC - Frozen (1:50 - 1:100)
- Western blot (1:100 - 1:1000)
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Type IV collagen isolated from human placenta
Recognizes the extracellular, basement membranes.
Methanol fixed cells are recommended for immunocytochemistry. A PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2 is recommended for immunohistochemistry on frozen tissues. For immunohistochemistry on paraffin tissue sections, the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature.
PBS, 0.09% Sodium Azide
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
For research use only.
This antibody carries the LSBio 100% Guarantee.