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Anti-CHEK2 / CHK2 Antibody (clone 73C175.1.1) IHC-plus™ LS-B1321

Note: This antibody replaces LS-C508

Ordering

Wt. Vol. Conc. Price
50 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CHEK2 / CHK2 Antibodies

Anti-CHEK2 / CHK2 Antibody LS-C123232
Rabbit Polyclonal (IgG) to Human CHEK2 / CHK2
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit
IHC, Western blot
Unconjugated
Anti-CHEK2 / CHK2 Antibody (clone 4B7) IHC-plus™ LS-B6212
Mouse Monoclonal [clone 4B7] (IgG2a,k) to Human CHEK2 / CHK2
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CHEK2 / CHK2 Antibody (phospho-Thr68) LS-C177890
Rabbit Polyclonal to Human CHEK2 / CHK2
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 73C175.1.1] (IgG1) to Human CHEK2 / CHK2
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human CHEK2 / CHK2
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [73C175.1.1]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (2 µg/ml)

Specificity and Use

CHEK2 / CHK2 antibody was raised against a synthetic peptide obtained from human Chk2 protein sequence.
A synthetic peptide obtained from human Chk2 protein sequence was used as immunogen.
Immunohistochemistry: LS-B1321 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1321 was determined to be 10 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Long term: -70°C; Short term: +4°C
For research use only.

About CHEK2 / CHK2

O96017 NM_007194 NP_009125.1

CHEK2 Antibody, BA444G7 Antibody, Cds1 homolog Antibody, Checkpoint kinase 2 Antibody, Checkpoint-like protein CHK2 Antibody, CHK2 checkpoint homolog Antibody, CHK2 Antibody, HCds1 Antibody, LFS2 Antibody, RAD53 Antibody, Rad53 homolog Antibody, HCHK2 Antibody, HuCds1 Antibody, PP1425 Antibody

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T].

Immunohistochemistry

Immunohistochemistry
Anti-CHEK2 / CHK2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1321 concentration 10 ug/ml.

Western blot

Western blot
Western blot of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with antibody at 2 ug/ml.

Requested From: United States
Date Requested: 12/10/2016

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