CDK4 antibody LS-C22050 is an unconjugated mouse monoclonal antibody to CDK4 from human, mouse, rat and other species. Validated for IP and WB.
Human, Mouse, Rat, Pig
(tested or 100% immunogen sequence identity)
Tissue culture supernatant
- Western blot
CDK4 antibody was raised against human recombinant full-length cdk4 polypeptide.
Species cross-reactivity: Human, porcine, rat, mouse. Reacts specifically with cdk4 protein present predominantly in G1 phase of cell cycle; it does not cross-react with cyclins. In immunohistochemical application on frozen sections of head and neck carcinoma (oropharynx and hypopharynx carcinoma) the antibody shows distinct nuclear staining, especially in tumor areas of enhanced proliferation.
Suitable for use in Immunoprecipitation and Western Blot (Western). Immunoprecipitation: Ready-to-use; Incubation Time 1-4 h at RT or overnight at 2-8?C.
0.09% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.