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Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
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order histories, retained contact details for faster checkout, review submissions, and special promotions.
CDK4 antibody LS-C22049 is an unconjugated mouse monoclonal antibody to CDK4 from human. It is reactive with human, mouse, rat and other species. Validated for Func, IF, IP and WB.
Human, Mouse, Rat, Pig
(tested or 100% immunogen sequence identity)
Clonality
IgG1,kMonoclonal
Clone
DCS-35
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Purified recombinant human cdk4 protein.
Specificity
Recognizes a 34kD protein identified as p34 or cyclin-dependent kinase 4 (cdk4). It shows no cross-reaction with other cdks. Species cross-reactivity: Human, mouse, rat and porcine.
Applications
Immunofluorescence
Western blot (1 - 2 µg/ml)
Immunoprecipitation
Functional Assay
Usage
Suitable for use in Western Blot, Immunofluorescence,. Immunoprecipitation and Kinase Assay. Western Blot: 1-2 ug/ml for 2 hrs at RT. Immunoprecipitation: Excellent for coprecipitation of D-type cyclins bound to cdk4.
Presentation
10 mM PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.