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Catalog Number Size Price
LS-C99873-400 400 µl $393 
CDK4 Antibody - Formalin-fixed and paraffin-embedded human prostate carcinoma with CDK4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
CDK4 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
CDK4 Antibody - Fluorescent image of HeLa cell stained with CDK4 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDK4 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). CDK4 immunoreactivity is localized to Cytoplasm significantly.
CDK4 Antibody - Western blot of CDK4 (arrow) using rabbit polyclonal CDK4 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDK4 gene (Lane 2) (Origene Technologies).
CDK4 Antibody - Western blot of anti-CDK4 antibody in HL-60 cell lysate. CDK4 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
CDK4 Antibody - CDK4 Antibody flow cytometry of HL60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CDK4 Antibody - Formalin-fixed and paraffin-embedded human prostate carcinoma with CDK4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
CDK4 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
CDK4 Antibody - Fluorescent image of HeLa cell stained with CDK4 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDK4 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). CDK4 immunoreactivity is localized to Cytoplasm significantly.
CDK4 Antibody - Western blot of CDK4 (arrow) using rabbit polyclonal CDK4 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDK4 gene (Lane 2) (Origene Technologies).
CDK4 Antibody - Western blot of anti-CDK4 antibody in HL-60 cell lysate. CDK4 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
CDK4 Antibody - CDK4 Antibody flow cytometry of HL60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Polyclonal Rabbit anti‑Human CDK4 Antibody (aa273‑305, IHC, IF, WB) LS‑C99873

Polyclonal Rabbit anti‑Human CDK4 Antibody (aa273‑305, IHC, IF, WB) LS‑C99873

Antibody:
CDK4 Rabbit anti-Human Polyclonal (aa273-305) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C99873-400
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CDK4 Rabbit anti-Human Polyclonal (aa273-305) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CDK4 antibody LS-C99873 is an unconjugated rabbit polyclonal antibody to human CDK4 (aa273-305). Validated for Flow, IF, IHC and WB.
Target
Human CDK4
Synonyms
CDK4 | Cell division protein kinase 4 | Crk3 | Cyclin-dependent kinase 4 | CMM3 | p34/cdk4 | PSK-J3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa273-305
Specificity
This CDK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-305 amino acids from the C-terminal region of human CDK4.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDK4
P11802 NM_000075 NP_000066.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 6/25/2024