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Anti-CDK1 / CDC2 Antibody (phospho-Tyr15) LS-C183

Note: This antibody replaces LS-C43069

Ordering

Wt. Vol. Conc. Price
- 100 µl - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CDK1 / CDC2 Antibodies

Anti-CDK1 / CDC2 Antibody (Thr161) IHC-plus™ LS-B1179
Rabbit Polyclonal (IgG) to Human CDK1 / CDC2
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CDK1 / CDC2 Antibody (Tyr15) IHC-plus™ LS-B1180
Rabbit Polyclonal (IgG) to Human CDK1 / CDC2
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CDK1 / CDC2 Antibody (clone 26E11) IHC-plus™ LS-B7092
Mouse Monoclonal [clone 26E11] (IgG2a,k) to Human CDK1 / CDC2
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Rat CDK1 / CDC2
Rat, Human, Mouse, Xenopus
Western blot
Unconjugated

Details

Rat CDK1 / CDC2
Rabbit
Rat, Human, Mouse, Xenopus (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

Western blot (1:1000)

Specificity and Use

CDK1 / CDC2 antibody was raised against phosphopeptide corresponding to amino acid residues surrounding the phosphoTyr1 of rat cdc2.
pTyr15
Specific for the ~38k cdc2 protein phosphorylated at Tyr15. Immunolabeling is blocked by the X-phosphatase treatment

Packaging

10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol
Long term: -20°C; Short term: -20°C
For research use only.

About CDK1 / CDC2

P06493 NM_001786 NP_001777.1

CDK1 Antibody, CDC28A Antibody, CDC2 Antibody, CDKN1 Antibody, Cyclin-dependent kinase 1 Antibody, p34 protein kinase Antibody, p34CDC2 Antibody, CDC2a Antibody, Cell cycle controller CDC2 Antibody, Cell division protein kinase 1 Antibody, PSTAIR Antibody

Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis.

Western blot

Western blot
Western blot of human T47D cells showing specific immunolabeling of the ~34k cdc2 phosphorylated at Tyr15 (Control). Treatment with EGF (30 ng per ml for 30 min) caused dephosphorylation of the Tyr15 on cdc2 (Lane 2). The phosphospecificity of this labeling is also shown in the third and fourth lanes (lambda-phosphatase: l-Ptase). These blots are identical to the control except that they were incubated in l-Ptase (1200 units for 30 min) before being exposed to the Anti-Phospho-Tyr15 cdc2. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
 

Requested From: United States
Date Requested: 12/8/2016

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