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Catalog Number Size Price
LS-C18772-100 100 µg (1 mg/ml) $485 
CDK1 / CDC2 Antibody - Mab anti-Human p34Cdc2 antibody was used to detect human p34Cdc2by Western blot of untreated (control) and drug treated (10 M genistein) lysates of MCF-7 cells. Very strong detection occurs using a 1:1000 dilution. Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center.
CDK1 / CDC2 Antibody - Mab anti-Human p34°Cdc2 antibody (clone POH-1) is shown to detect human p34°Cdc2by western blot. Detection occurs after 10 ug of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1000 dilution of Mab anti-Human p34°Cdc2at room temperature for 30 min followed by detection using IRDye800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:5000. A doublet band corresponding to human p34°Cdc2is detected at ~34 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human p34°Cdc2. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
CDK1 / CDC2 Antibody - Mab anti-Human p34cdc2 antibody was used at a dilution of 1:1000 to detect human p34cdc2by Western blot of untreated (control) and drug treated (10 mM genistein) lysates of MCF-7 cells.
CDK1 / CDC2 Antibody - Mab anti-Human p34Cdc2 antibody was used to detect human p34Cdc2by Western blot of untreated (control) and drug treated (10 M genistein) lysates of MCF-7 cells. Very strong detection occurs using a 1:1000 dilution. Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center.
CDK1 / CDC2 Antibody - Mab anti-Human p34°Cdc2 antibody (clone POH-1) is shown to detect human p34°Cdc2by western blot. Detection occurs after 10 ug of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1000 dilution of Mab anti-Human p34°Cdc2at room temperature for 30 min followed by detection using IRDye800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:5000. A doublet band corresponding to human p34°Cdc2is detected at ~34 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human p34°Cdc2. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
CDK1 / CDC2 Antibody - Mab anti-Human p34cdc2 antibody was used at a dilution of 1:1000 to detect human p34cdc2by Western blot of untreated (control) and drug treated (10 mM genistein) lysates of MCF-7 cells.
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Monoclonal Mouse anti‑Human CDK1 / CDC2 Antibody (clone POH‑1, IHC, WB) LS‑C18772

Monoclonal Mouse anti‑Human CDK1 / CDC2 Antibody (clone POH‑1, IHC, WB) LS‑C18772

Antibody:
CDK1 / CDC2 Mouse anti-Human Monoclonal (POH-1) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-C18772-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CDK1 / CDC2 Mouse anti-Human Monoclonal (POH-1) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CDC2 antibody LS-C18772 is an unconjugated mouse monoclonal antibody to CDC2 (CDK1) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC, IP and WB. Cited in 3 publications.
Target
Human CDK1 / CDC2
Synonyms
CDK1 | CDC28A | CDC2 | CDKN1 | Cyclin-dependent kinase 1 | p34 protein kinase | p34CDC2 | CDC2a | Cell cycle controller CDC2 | Cell division protein kinase 1 | PSTAIR
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
POH-1
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p34 cdc2 fusion protein.
Specificity
Reacts specifically with p34 cdc2 in human tissues and cell lines. This antibody is not cross reactive with other cyclin dependent kinases. Cross reactivity with p34 cdc2 from other sources, especially mouse and rat will occur. This reagent has broad interspecies reactivity.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This antibody is suitable for immunohistochemistry immunoprecipitation (as active kinase), and immunoblotting. The antibody detects the three bands within the 34kD region corresponding to the p34 protein and its cleavage products. HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting. LEP fibroblast cell lysate is suggested as a negative control. Paraffin embedded tissue is reactive for immunohistochemistry using high temperature release and 0.1 % saponin treatment or other permeabilization method.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDK1 / CDC2
P06493 NM_001786 NP_001777.1

LSBio Ratings

CDK1 / CDC2 Antibody (clone POH-1) for IHC, WB/Western, IP, ELISA LS-C18772 has an LSBio Rating of
Publications (4.2)

Publications (3)

Distinct forms of human CDC2 identified by novel monoclonal antibodies. Luks J, Draetta G, Bartek J. European journal of biochemistry / FEBS. 1992 207:169-76.

Proteolysis and tyrosine phosphorylation of p34cdc2/cyclin B. The role of MCM2 and initiation of DNA replication to allow tyrosine phosphorylation of p34cdc2. Ye XS, Fincher RR, Tang A, McNeal KK, Gygax SE, Wexler AN, Ryan KB, James SW, Osmani SA. The Journal of biological chemistry. 1997 272:33384-93.

A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1. Palmer A, Gavin AC, Nebreda AR. The EMBO journal. 1998 17:5037-47.

Customer Reviews (0)


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Requested From: United States
Date Requested: 9/24/2021