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Anti-CDK1 / CDC2 Antibody (clone 8F1) LS-C133065

Catalog	Size	Price
LS-C133065-100	100 µg	Unavailable

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Antibody:	Mouse Monoclonal [clone 8F1] (IgG2a,l) to Human CDK1 / CDC2
Reactivity:	Human, Mouse, Rat
Application:	IHC - Paraffin, Western blot, ELISA
Format:	Unconjugated

Details

Target:	Human CDK1 / CDC2
Host:	Mouse
Reactivity:	Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality:	IgG2a,l Monoclonal [8F1]
Conjugations:	Unconjugated
Purification:	Purified from ascites by Protein A
Modifications:	Unmodified

Applications

Approved:

IHC - Paraffin (1.5 µg/ml)
Western blot
ELISA

Specificity and Use

Immunogen:	CDK1 / CDC2 antibody was raised against cDC2 (AAH14563, 211 a.a. ~ 298 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity:	Human CDC2
Usage:	Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Cell lysate, Cell lysate, Recombinant protein.

Packaging

Presentation:	PBS, pH 7.2
Storage:	Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Restrictions:	For research use only.

About CDK1 / CDC2

Uniprot: P06493 NCBI: NM_001786 NP_001777.1

Synonyms: CDK1 Antibody, CDC28A Antibody, CDC2 Antibody, CDKN1 Antibody, Cyclin-dependent kinase 1 Antibody, p34 protein kinase Antibody, p34CDC2 Antibody, CDC2a Antibody, Cell cycle controller CDC2 Antibody, Cell division protein kinase 1 Antibody, PSTAIR Antibody

Description: Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis.

(More About CDK1 / CDC2)

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Immunohistochemistry

Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1.5 ug/ml]

Western blot

CDC2 monoclonal antibody (M04), clone 8F1. Western blot of CDC2 expression in NIH/3T3.

Western blot

CDC2 monoclonal antibody (M04), clone 8F1. Western blot of CDC2 expression in Raw 264.7.

Western blot

CDC2 monoclonal antibody (M04), clone 8F1. Western blot of CDC2 expression in PC-12.

Western blot

CDC2 monoclonal antibody (M04), clone 8F1 Western blot of CDC2 expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CDC2 is approximately 0.1 ng/ml as a capture antibody.

Requested From:
Date Requested: 6/22/2017

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