Human CDH11 / Cadherin 11

Mouse

Human, Mouse (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

5B2H5

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Recombinant protein consisting of intracellular domain of human cadherin 11.

Intracellular

Recognizes the ~110kD human Cadherin 11 protein. Cross-reactivity with other cadherin family members has not been observed.

• IHC
• IHC - Paraffin (5 - 10 µg/ml)
• IHC - Frozen (5 - 10 µg/ml)
• Western blot (2 - 4 µg/ml)
• Immunoprecipitation (5 µg/ml)
• ELISA

Immunohistochemistry: LS-B2307 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2307 was determined to be 5-10 ug/ml. Immunoprecipitation: 5 ug. Native, human; Not tested for mouse. Positive control: MDA. MB231 cells (human) and mouse L fibroblast lysates.

PBS, pH 7.4, 0.09% Sodium Azide

Short term 4°C (no longer than 1 week); Long term -20°C; Aliquot to avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P55287 NCBI: NM_001797 NP_001788.2