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Anti-CD9 Antibody (clone HI9a) IHC-plus™ LS-B1964

Note: This antibody replaces LS-C41006

Catalog	Size	Price
LS-B1964-50	50 µg (0.5 mg/ml)	Unavailable

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Anti-CD9 Antibody (clone HI9a) IHC-plus™ LS-B1964

Antibody:	Mouse Monoclonal [clone HI9a] (IgG1,k) to Human CD9
Reactivity:	Human, Bovine, Dog, Horse, Rabbit, Sheep
Application:	IHC, IHC - Paraffin, Flow Cytometry
Format:	Unconjugated

Anti-CD9 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1964 concentration 10 ug/ml.

Anti-CD9 Antibody (clone SN4/C3-3A2, Biotin) LS-C105919

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$Staining of normal human platelets with 0.25 ug of Biotin Mouse IgG1, K isotype control (open histogram) or 0.25\ug Biotin anti-human CD9 (SN4) (colored histogram) followed by PE-SAv. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.$

Anti-CD9 Antibody (clone SN4/C3-3A2) IHC-plus™ LS-B4042

Antibody:	Mouse Monoclonal [clone SN4/C3-3A2] (IgG1) to Human CD9
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Anti-CD9 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4042 concentration 20 ug/ml.

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Immunofluorescence of monoclonal antibody to CD9 on HeLa cell . [antibody concentration 10 ug/ml]

Antibody:	Mouse Monoclonal [clone HI9a] (IgG1,k) to Human CD9
Reactivity:	Human, Bovine, Dog, Horse, Rabbit, Sheep
Application:	IHC, IHC - Paraffin, Flow Cytometry
Format:	Unconjugated

Human platelets stained with purified HI9a, then detected with anti-mouse IgGs FITC.

Details

Target:	Human CD9
Host:	Mouse
Reactivity:	Human, Bovine, Dog, Horse, Rabbit, Sheep (tested or 100% immunogen sequence identity)
Clonality:	IgG1,k Monoclonal [HI9a]
Conjugations:	Unconjugated
Purification:	Affinity purified
Modifications:	Unmodified

Applications

Approved:

IHC
IHC - Paraffin (10 µg/ml)
Flow Cytometry (0.5 µg/10E6 cells)

Specificity and Use

Usage:

Immunohistochemistry: LS-B1964 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1964 was determined to be 10 ug/ml.

Packaging

Presentation:	PBS, pH 7.2, 0.09% sodium azide.
Storage:	+4°C. Store undiluted.
Restrictions:	For research use only.

About CD9

Uniprot: P21926 NCBI: NM_001769 NP_001760.1

Synonyms: CD9 Antibody, 5H9 antigen Antibody, BA2 Antibody, BA-2/p24 antigen Antibody, CD9 antigen Antibody, CD9 antigen (p24) Antibody, DRAP-27 Antibody, MIC3 Antibody, MRP-1 Antibody, Leukocyte antigen MIC3 Antibody, Tetraspanin-29 Antibody, TSPAN29 Antibody, BTCC-1 Antibody, CD9 molecule Antibody, Motility related protein-1 Antibody, Motility-related protein Antibody, TSPAN-29 Antibody

Description: CD9 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis.

(More About CD9)

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Immunohistochemistry

Anti-CD9 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1964 concentration 10 ug/ml.

Flow Cytometry

Human platelets stained with purified HI9a, then detected with anti-mouse IgGs FITC.

Requested From:
Date Requested: 4/20/2018

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