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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD9

CD9 molecule

CD9 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis.

Gene Name: CD9 molecule
Family/Subfamily: Tetraspan , not assigned-Tetraspan
Synonyms: CD9, 5H9 antigen, BA2, BA-2/p24 antigen, CD9 antigen, CD9 antigen (p24), DRAP-27, MIC3, MRP-1, Leukocyte antigen MIC3, Tetraspanin-29, TSPAN29, BTCC-1, CD9 molecule, Motility related protein-1, Motility-related protein, TSPAN-29
Target Sequences: NM_001769 NP_001760.1 P21926

Publications (6)

1
Potentials and capabilities of the Extracellular Vesicle (EV) Array. Jørgensen MM, Bæk R, Varming K. Journal of Extracellular Vesicles. 2015 4:26048. (IHC; Human) [PubMed:25862471] [PMC:PMC4393420]
2
Characterization of a Cell-Culturing System for the Study of Contact-Independent Extracellular Vesicle Communication. Revenfeld ALS, Søndergaard EKL, Stensballe A, Bæk R, Jørgensen MM, Varming K. Journal of circulating biomarkers. 2016 February;5:3. (Human) [Full Text Article] [PubMed:28936251] [PMC:PMC5548315]
3
Induction of a Regulatory Phenotype in CD3+ CD4+ HLA-DR+ T Cells after Allogeneic Mixed Lymphocyte Culture; Indications of Both Contact-Dependent and -Independent Activation. Revenfeld ALS, Bæk R, Jørgensen MM, Varming K, Stensballe A. International journal of molecular sciences. 2017 July;18:pii: E1603. (Human) [Full Text Article] [PubMed:28737722] [PMC:PMC5536089]
4
CD9-positive microvesicles mediate the transfer of molecules to Bovine Spermatozoa during epididymal maturation. Caballero JN, Frenette G, Belleann[Character e9]e C, Sullivan R. PloS one. 2013 8:e65364. [Full Text Article] [PubMed:23785420] [PMC:PMC3681974] Related Antibodies: LS-B5962.
5
Effects of pramipexole treatment on the α-synuclein content in serum exosomes of Parkinson's disease patients. Luo HT, Zhang JP, Miao F. Experimental and therapeutic medicine. 2016 12:1373-1376. (IHC, WB; Human) [Full Text Article] [PubMed:27588058] [PMC:PMC4998109] Related Antibodies: LS-C382578.
6
Extracellular Vesicle (EV) Array: microarray capturing of exosomes and other extracellular vesicles for multiplexed phenotyping. Jorgensen M1, Baek R, Pedersen S, Sondergaard EK, Kristensen SR, Varming K. Journal of Extracellular Vesicles. 2013 Jun 18;2: [Full Text Article] [PubMed:24009888] [PMC:PMC3760630] Related Antibodies: LS-C35419.
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Filtered by: None
Target
CD9 (144)
Reactivity
Human (126)
Mouse (32)
Rat (15)
Bat (4)
Bovine (18)
Cat (9)
Chimpanzee (3)
Dog (10)
Gibbon (3)
Guinea pig (4)
Horse (10)
Monkey (14)
Pig (9)
Rabbit (7)
Sheep (7)
Application
IHC (42)
IHC-Fr (11)
IHC-P (16)
WB (80)
Flo (102)
ELISA (18)
Func (12)
ICC (7)
IF (15)
IP (25)
Peptide-ELISA (2)
Host
rabbit (39)
mouse (90)
rat (15)
Isotype
IgG (19)
IgG1 (34)
IgG1,k (21)
IgG2 (2)
IgG2a (9)
IgG2a,k (4)
IgG2b (32)
Clonality
monoclonal mc (106)
polyclonal pc (38)
Clone
10H6 (2)
4A2 (1)
5G6 (2)
Bu16 (2)
CLB-47 (1)
EM-04 (9)
HI9a (6)
IVA50 (2)
JF9 (2)
K41 (1)
KMC8 (3)
MEM-61 (9)
MF1 (1)
MM2/57 (14)
SN4/C3-3A2 (9)
Format
AF647 Conjugated (1)
AP Conjugated (5)
APC (1)
APC Conjugated (10)
Azide-free (6)
Biotin Conjugated (15)
Concentrated (1)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (22)
HRP Conjugated (7)
Low Endotoxin (1)
PE Conjugated (19)
PerCP Conjugated (1)
RPE Conjugated (2)
Unconjugated (60)
Epitope
aa112-195 (8)
aa21-51 (8)
Extracellular Domain (5)
N-Terminus (4)
Internal (2)
70-150 aa, Internal (1)
Tyr129 (1)
aa101-150 (1)
aa80-129 (1)
Publications
No (141)
Yes (3)
Showing 1-25 of 144 results

IHC-plus™ CD9 Antibody (clone MM2/57) LS-B5962
Publications (1)
Publications (1)
Mouse Monoclonal [clone MM2/57] (IgG2b) to Human CD9
Human
Flo, IHC, IHC-Fr, IHC-P, WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-CD9 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ CD9 Antibody (clone HI9a) LS-B1964
Mouse Monoclonal [clone HI9a] (IgG1,k) to Human CD9
Rabbit, Dog, Sheep, Bovine, Horse, Human
Flo, IHC, IHC-P
Unconjugated
50µg 0.5mg/ml  $425.00
Anti-CD9 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Human platelets stained with purified HI9a, then detected with anti-mouse IgGs FITC.

CD9 Antibody (70-150 aa, Internal) LS-C382578
Publications (1)
Publications (1)
Rabbit Polyclonal (IgG) to Human CD9
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of CD9 antibody
Western blot of CD9 antibody

CD9 Antibody (clone SN4/C3-3A2, Biotin) LS-C35419
Publications (1)
Publications (1)
Mouse Monoclonal [clone SN4/C3-3A2] (IgG1,k) to Human CD9
Human
Flo
Biotin Conjugated
100µg 1mg/ml  $495.00

IHC-plus™ CD9 Antibody (clone SN4/C3-3A2) LS-B4042
Mouse Monoclonal [clone SN4/C3-3A2] (IgG1) to Human CD9
Human
Flo, IHC, IHC-Fr, IHC-P
Unconjugated
50µg 0.5mg/ml  $425.00
Anti-CD9 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Anti-CD9 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

CD9 Antibody (clone SN4/C3-3A2, Biotin) LS-C105919
Mouse Monoclonal [clone SN4/C3-3A2] (IgG1) to Human CD9
Human
Flo
Biotin Conjugated
25µg  $215.00; 100µg 0.5mg/ml  $275.00
Staining of normal human platelets with 0.25 ug of Biotin Mouse IgG1, K isotype control (open histogram) or 0.25\ug Biotin anti-human CD9 (SN4) (colored histogram) followed by PE-SAv. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

CD9 Antibody (clone 4A2) LS-C196846
Mouse Monoclonal [clone 4A2] (IgG2a,k) to Human CD9
Human
ELISA, IF, WB
Unconjugated
100µg 0.42mg/ml  $365.00
Immunofluorescence of monoclonal antibody to CD9 on HeLa cell . [antibody concentration 10 ug/ml]
Detection limit for recombinant GST tagged CD9 is approximately 1 ng/ml as a capture antibody.

CD9 Antibody (Internal) LS-C354601
Rabbit Polyclonal to Human CD9
Mouse, Rat, Human, Monkey
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of CD9 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. w
Immunofluorescent analysis of CD9 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

CD9 Antibody (clone EM-04) LS-C112425
Rat Monoclonal [clone EM-04] (IgG1) to Mouse CD9
Mouse
Flo, ICC, IP, WB
Unconjugated
0.1mg 1mg/ml  $225.00
Surface staining of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) purified, DAR-APC.

CD9 Antibody (N-Terminus) LS-C135937
Rabbit Polyclonal to Human CD9
Rabbit, Guinea pig, Sheep, Bovine, Rat, Bat, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $385.00
CD9 antibody Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

CD9 Antibody (N-Terminus, HRP) LS-C468355
Rabbit Polyclonal to Human CD9
Rabbit, Guinea pig, Sheep, Bovine, Rat, Bat, Gibbon, Chimpanzee, Human, Monkey
WB
HRP Conjugated
100µl 0.65mg/ml  $405.00
CD9 antibody Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

CD9 Antibody (clone MM2/57, Azide-free, Low Endotoxin) LS-C351454
Mouse Monoclonal [clone MM2/57] (IgG2b) to Human CD9
Human
Flo, IHC, IHC-Fr, IP, WB
Unconjugated, Azide-free, Low Endotoxin
0.5mg 0.5mg/ml  $515.00

CD9 Antibody (Tyr129) LS-C177343
Rabbit Polyclonal to Human CD9
Mouse, Rat, Human
IF, IHC, IHC-P, WB
Unconjugated
100µl  $345.00
IHC of CD9 (Y129) pAb in paraffin-embedded human breast carcinoma tissue.
Western blot of CD9 (Y129) pAb in extracts from rat heart.

CD9 Antibody LS-C416960
Rabbit Polyclonal to Human CD9
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
100µg  $305.00
Western blot of CD9 expression in COLO cells

CD9 Antibody (clone IVA50) LS-C46004
Mouse Monoclonal [clone IVA50] (IgG2) to Bovine CD9
Bovine
Flo, Func, IP, WB
Unconjugated
0.1mg 1mg/ml  $255.00

CD9 Antibody (clone KMC8) LS-C106704
Rat Monoclonal [clone KMC8] (IgG2a,k) to Mouse CD9
Mouse
Flo, WB
Unconjugated
50µg  $205.00; 100µg 0.5mg/ml  $245.00; 500µg  $345.00
Staining of C57Bl/6 bone marrow cells with 0.5 ug of Purified Rat IgG2a isotype control (open histogram) or 0.5 ug of Purified anti-mouse CD9 (KMC8) (colored histogram) followed by PE Anti-Rat IgG (LS-C106534). Cells in the large scatter population were used for analysis.

CD9 Antibody LS-C675301
Rabbit Monoclonal (IgG) to Human CD9
Human
ELISA, Flo, IF, IHC, IHC-P, WB
Unconjugated
50µl  $315.00; 100µl  $425.00
Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of MCF-7 cells diluted at 1:34,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

CD9 Antibody (clone 5G6) LS-C169447
Mouse Monoclonal [clone 5G6] (IgG1) to Human CD9
Human
ELISA, Flo, IHC, IHC-P, WB
Unconjugated
100µl  $365.00
IHC of paraffin-embedded cervical cancer tissues using CD9 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded kidney cancer tissues using CD9 mouse monoclonal antibody with DAB staining.

CD9 Antibody (aa101-150) LS-C119436
Rabbit Polyclonal (IgG) to Human CD9
Mouse, Rat, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CD9 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using CD9 Antibody. The picture on the right is blocked with the synthesized peptide.

CD9 Antibody LS-C661808
Rabbit Polyclonal (IgG) to Human CD9
Human
ELISA, Flo, IHC, WB
Unconjugated
100µg 2mg/ml  $405.00

CD9 Antibody (clone Bu16) LS-C188356
Mouse Monoclonal [clone Bu16] (IgG2a) to Human CD9
Human
Flo, IF, IP, WB
Unconjugated
0.2mg  $395.00
Flow cytometry of human peripheral blood platelets with Mouse anti-Human CD9

CD9 Antibody (clone EM-04, Biotin) LS-C204846
Rat Monoclonal [clone EM-04] (IgG1) to Mouse CD9
Mouse
Flo, ICC, IP, WB
Biotin Conjugated
0.1mg 1mg/ml  $235.00

CD9 Antibody (PE) LS-C4078
Mouse Monoclonal (IgG2b) to Human CD9
Human
Flo, Func, IHC, IHC-Fr, IP, WB
PE Conjugated
100tst  $495.00

CD9 Antibody (clone MF1) LS-C188404
Rat Monoclonal [clone MF1] (IgG2b) to Mouse CD9
Mouse
Flo, IP, WB
Unconjugated
100µg 1mg/ml  $295.00; 0.25mg  $365.00
Flow cytometry of Mouse Peripheral Platelets with Rat anti-Mouse CD9

CD9 Antibody (aa21-51) LS-C320217
Rabbit Polyclonal (IgG) to Human CD9
Human
ELISA, Flo, WB
Unconjugated
200µl  $590.00
Showing 1-25 of 144 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).