Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry (applications tested for the base form of this product only)
Human (tested or 100% immunogen sequence identity)
Also available conjugated with FITC, RPE.
Protein G purified
IHC - Paraffin (10 µg/ml)
IHC - Frozen
Flow Cytometry (1:50 - 1:100)
(applications tested for the base form of this product only)
Specificity and Use
CD59 antibody was raised against human peripheral blood T cells.
Recognizes the human CD59 cell surface antigen, an 18-20kD GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes. Clone YTH53.1 has been shown to block the normal function of CD59. Removal of sodium azide is recommended prior to use in functional assays. XXX recommend the use of EQU003 for this purpose. CD59 is expressed by erythrocytes, and therefore staining in whole blood systems requires a higher concentration of antibody.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.
PBS, 0.09% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
CD59 is a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation.
Anti-CD59 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5245 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CD59 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5245 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.