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Anti-CD47 Antibody (Intracellular Domain, clone EPR4150(2)) IHC-plus™ LS-B8451

Note: This antibody replaces LS-C138614
See our new CD47 ELISA Kits
Human CD47 ELISA Kit - LS-F7016
(kits do not necessarily contain the antibody listed on this page)

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

CD47 Antibody LS-B8451 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-CD47 antibody IHC of human prostate, epithelium.
Western blot
Western blot analysis on (A) Jurkat, (B) HeLa, and (C) K562 cell lysates using anti-CD47 antibody.
Flow Cytometry
Flow cytometry of permeabilized Jurkat cells using anti-CD47 antibody (red)or a rabbit IgG...

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Rabbit Monoclonal [clone EPR4150(2)] to Human CD47
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot, Flow Cytometry
Unconjugated

Details

Human CD47
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Monoclonal [EPR4150(2)]
Tissue culture supernatant

Applications

IHC - Paraffin (1:50), ICC (1:100 - 1:250), Western blot (1:1000 - 1:10000), Flow Cytometry (1:100 - 1:500)

Specificity and Use

CD47 antibody was raised against synthetic peptide
Intracellular Domain
Human CD47. Species cross-reactivity is based on WB analysis.

Packaging

Tris-Glycine-NaCl or PBS, pH 7.2-7.4, 40-50% Glycerol, 0.01% sodium azide, 0.05% BSA
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About CD47

Q08722 NM_001777 NP_001768.1

CD47 Antibody, CD47 antigen Antibody, CD47 molecule Antibody, Integrin associated protein Antibody, Integrin-associated protein Antibody, Leukocyte surface antigen CD47 Antibody, MER6 Antibody, Protein MER6 Antibody, Rh-related antigen Antibody, OA3 Antibody, CD47 glycoprotein Antibody, IAP Antibody

CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, and is reduced in expression on Rh erythrocytes.

Immunohistochemistry

Immunohistochemistry
Anti-CD47 antibody IHC of human prostate, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8451 dilution 1:50.

Western blot

Western blot
Western blot analysis on (A) Jurkat, (B) HeLa, and (C) K562 cell lysates using anti-CD47 antibody.

Flow Cytometry

Flow Cytometry
Flow cytometry of permeabilized Jurkat cells using anti-CD47 antibody (red)or a rabbit IgG (negative) (green).
 
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Requested From: United States
Date Requested: 5/22/2015

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