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Catalog Number Size Price
LS-C357794-20 20 µg (0.2 mg/ml) $347 
LS-C357794-100 100 µg (0.2 mg/ml) $522 
CD47 Antibody - Western blot testing of human brain lysate with CD47 antibody (clone B6H12.2). Predicted molecular weight ~35kDa but observed at 35-60kDa depending on glycosylation level.

Monoclonal Mouse anti‑Human CD47 Antibody (clone B6H12.2, IHC, IF, WB) LS‑C357794

Monoclonal Mouse anti‑Human CD47 Antibody (clone B6H12.2, IHC, IF, WB) LS‑C357794

Antibody:
CD47 Mouse anti-Human Monoclonal (B6H12.2) Antibody
Application:
IHC, IHC-P, IF, WB, Flo, Block, Func
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$347
LS-C357794-20
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CD47 Mouse anti-Human Monoclonal (B6H12.2) Antibody
Application:
IHC, IHC-P, IF, WB, Flo, Block, Func
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CD47 antibody LS-C357794 is an unconjugated mouse monoclonal antibody to human CD47. Validated for Block, Flow, Func, IF, IHC and WB.
Target
Human CD47
Synonyms
CD47 | CD47 antigen | CD47 molecule | Integrin associated protein | Integrin-associated protein | Leukocyte surface antigen CD47 | MER6 | Protein MER6 | Rh-related antigen | OA3 | CD47 glycoprotein | IAP
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
B6H12.2
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified. Also available Azide-free, BSA-free.
Immunogen
Intact CD47 purified from placenta.
Specificity
Human CD47
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (1 - 2 µg/ml)
  • Western blot
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • Blocking
  • Functional Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This antibody reacts with the Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. Functional Activity/blocking: Order BSA/Azide-free format
Presentation
1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD47
Q08722 NM_001777 NP_001768.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/4/2021