Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C782296-100 100 µg $575 
CD47 Antibody - IHC staining of FFPE human mouse brain with CD47 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
CD47 Antibody - IHC staining of FFPE human rat brain with CD47 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
CD47 Antibody - IHC staining of FFPE human mouse brain with CD47 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
CD47 Antibody - IHC staining of FFPE human tonsil tissue with CD47 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
CD47 Antibody - IHC staining of FFPE human ovarian cancer with CD47 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
CD47 Antibody - IF/ICC staining of FFPE human A431 cells with CD47 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
CD47 Antibody - Western blot testing of 1) rat brain, 2) rat kidney and 3) mouse brain lysate with CD47 antibody at 0.5ug/ml. Predicted molecular weight: 35~60 kDa depending on glycosylation level.
CD47 Antibody - IHC staining of FFPE human mouse brain with CD47 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
CD47 Antibody - IHC staining of FFPE human rat brain with CD47 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
CD47 Antibody - IHC staining of FFPE human mouse brain with CD47 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
CD47 Antibody - IHC staining of FFPE human tonsil tissue with CD47 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
CD47 Antibody - IHC staining of FFPE human ovarian cancer with CD47 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
CD47 Antibody - IF/ICC staining of FFPE human A431 cells with CD47 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
CD47 Antibody - Western blot testing of 1) rat brain, 2) rat kidney and 3) mouse brain lysate with CD47 antibody at 0.5ug/ml. Predicted molecular weight: 35~60 kDa depending on glycosylation level.
1 of 7
2 of 7
3 of 7
4 of 7
5 of 7
6 of 7
7 of 7

Polyclonal Rabbit anti‑Human CD47 Antibody (IHC, IF, WB) LS‑C782296

Polyclonal Rabbit anti‑Human CD47 Antibody (IHC, IF, WB) LS‑C782296

Antibody:
CD47 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$575
LS-C782296-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD47 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CD47 antibody LS-C782296 is an unconjugated rabbit polyclonal antibody to CD47 from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IF, IHC and WB.
Target
Human CD47
Synonyms
CD47 | CD47 antigen | CD47 molecule | Integrin associated protein | Integrin-associated protein | Leukocyte surface antigen CD47 | MER6 | Protein MER6 | Rh-related antigen | OA3 | CD47 glycoprotein | IAP
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antigen Affinity purification
Modifications
Unmodified
Immunogen
Amino acids Q19-E323 from the human protein were used as the immunogen for the CD47 antibody.
Applications
  • IHC - Paraffin (1 - 2 µg/ml)
  • Immunofluorescence (2 - 4 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • Flow Cytometry (1 - 3 µg/10E6 cells)
  • ELISA (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Optimal dilution of the CD47 antibody should be determined by the researcher.
Presentation
Lyophilized from PBS, 2% Trehalose, 0.025% sodium azide
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, store at 4°C for up to 1 month. Long-term: aliquot and store at -20°C. Avoid freeze-thaws cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD47
Q08722 NM_001777 NP_001768.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/6/2024