Target
Human CD4
Synonyms
CD4 | CD4 antigen | CD4 receptor | CD4mut | CD4 antigen (p55) | CD4 molecule
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Phycoerythrin, Cy7.
Also available
Unconjugated or conjugated with
Biotin,
FITC,
RPE,
APC.
Purification
Protein A purified
Immunogen
CD4+ transfectant/human CEM
Specificity
Antibody QS4120 recognizes the domain 1 region of the CD4 molecule (1, 2).
Applications
- Flow Cytometry
- ELISA
- Blocking
- Applications tested for the base form of this product only
Usage
Reagent was tested for binding to ficoll prepared human peripheral blood mononuclear cells (PBMC) in FACS. Five x 105 PBMC per tube were washed and pre incubated with 20 ul of 300 ug/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of antihuman CD4/PE-Cy7 at a 1:50 dilution (5 ug/ml). Cells were then washed three times, fixed and analyzed by FACS using a lymphoid gate. A 41% sub population of the cells stained positive with a mean shift of 2.0 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-human CD4 antibody. ELISA: Use for capture. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 500 mM KCl, 0.04% Sodium Azide, 15% Glycerol, 0.2% BSA
Storage
Store at 4°C. Do not freeze. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee